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New page: left|200px<br /><applet load="2oqu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oqu, resolution 1.80Å" /> '''High Pressure Cryoco...
 
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[[Image:2oqu.jpg|left|200px]]<br /><applet load="2oqu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2oqu, resolution 1.80&Aring;" />
'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''<br />


==Overview==
==High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths==
Crystal cryocooling is usually employed to reduce radiation damage during, X-ray crystallography. Recently, a high-pressure cryocooling method has, been developed which results in excellent diffraction-quality crystals, without the use of penetrative cryoprotectants. Three new developments of, the method are presented here: (i) Xe-He high-pressure cryocooling for Xe, SAD phasing, (ii) native sulfur SAD phasing and (iii) successful, cryopreservation of crystals in thick-walled capillaries without, additional cryoprotectants other than the native mother liquor. These, developments may be useful for structural solution of proteins without the, need for selenomethionine incorporation and for high-throughput protein, crystallography.
<StructureSection load='2oqu' size='340' side='right'caption='[[2oqu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2oqu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OQU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqu OCA], [https://pdbe.org/2oqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oqu RCSB], [https://www.ebi.ac.uk/pdbsum/2oqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oqu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG] Acts upon elastin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oq/2oqu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oqu ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.


==About this Structure==
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths.,Kim CU, Hao Q, Gruner SM Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791<ref>PMID:17452791</ref>
2OQU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CA, SO4 and XE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OQU OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17452791 17452791]
</div>
[[Category: Pancreatic elastase]]
<div class="pdbe-citations 2oqu" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
 
==See Also==
*[[Elastase 3D structures|Elastase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Gruner, S.M.]]
[[Category: Gruner SM]]
[[Category: Hao, Q.]]
[[Category: Hao Q]]
[[Category: Kim, C.U.]]
[[Category: Kim CU]]
[[Category: CA]]
[[Category: SO4]]
[[Category: XE]]
[[Category: high pressure cryocooling]]
[[Category: sad phasing]]
[[Category: xenon]]
 
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Retrieved from "https://proteopedia.org/2oqu"