2ote: Difference between revisions
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New page: left|200px<br /><applet load="2ote" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ote, resolution 1.47Å" /> '''Crystal structure of... |
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== | ==Crystal structure of a monomeric cyan fluorescent protein in the photobleached state== | ||
Fluorescent protein (FP) variants that can be reversibly converted between | <StructureSection load='2ote' size='340' side='right'caption='[[2ote]], [[Resolution|resolution]] 1.47Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ote]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clavularia_sp. Clavularia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OTE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.47Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ote FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ote OCA], [https://pdbe.org/2ote PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ote RCSB], [https://www.ebi.ac.uk/pdbsum/2ote PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ote ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/GFPL_CLASP GFPL_CLASP] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/2ote_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ote ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date. | |||
Structural basis for reversible photobleaching of a green fluorescent protein homologue.,Henderson JN, Ai HW, Campbell RE, Remington SJ Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458<ref>PMID:17420458</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2ote" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clavularia sp]] | |||
[[Category: Large Structures]] | |||
[[Category: Ai H]] | |||
[[Category: Campbell RE]] | |||
[[Category: Henderson JN]] | |||
[[Category: Remington SJ]] | |||
Latest revision as of 08:04, 19 March 2026
Crystal structure of a monomeric cyan fluorescent protein in the photobleached state
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