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| [[Image:2ow8.gif|left|200px]]<br /><applet load="2ow8" size="450" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[4v4i]] This PDB entry is obsolete and replaced by 4v4i |
| caption="2ow8, resolution 3.710Å" />
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| '''Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. This file, 2OW8, contains the 30S ribosome subunit, two tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1VSA.'''<br />
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| ==Overview==
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| Our understanding of the mechanism of protein synthesis has undergone, rapid progress in recent years as a result of low-resolution X-ray and, cryo-EM structures of ribosome functional complexes and high-resolution, structures of ribosomal subunits and vacant ribosomes. Here, we present, the crystal structure of the Thermus thermophilus 70S ribosome containing, a model mRNA and two tRNAs at 3.7 A resolution. Many structural details of, the interactions between the ribosome, tRNA, and mRNA in the P and E sites, and the ways in which tRNA structure is distorted by its interactions with, the ribosome are seen. Differences between the conformations of vacant and, tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure, in response to tRNA binding, including significant changes in the, peptidyl-transferase catalytic site.
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| ==About this Structure==
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| 2OW8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure superseeds the now removed PDB entry 2I1C. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OW8 OCA].
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| ==Reference==
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| Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements., Korostelev A, Trakhanov S, Laurberg M, Noller HF, Cell. 2006 Sep 22;126(6):1065-77. Epub 2006 Sep 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962654 16962654]
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| [[Category: Escherichia coli]]
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| [[Category: Protein complex]]
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| [[Category: Thermus thermophilus]]
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| [[Category: Korostelev, A.]]
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| [[Category: Laurberg, M.]]
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| [[Category: Noller, H.F.]]
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| [[Category: Trakhanov, S.]]
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| [[Category: crystal structure]]
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| [[Category: e site]]
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| [[Category: functional complex]]
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| [[Category: p site]]
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| [[Category: peptidyl transferase center]]
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| [[Category: ribosome]]
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| [[Category: trna]]
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| ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:19:48 2007''
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