2kbt: Difference between revisions

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{{Seed}}
[[Image:2kbt.png|left|200px]]


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==Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method==
The line below this paragraph, containing "STRUCTURE_2kbt", creates the "Structure Box" on the page.
<StructureSection load='2kbt' size='340' side='right'caption='[[2kbt]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2kbt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus], [https://en.wikipedia.org/wiki/Streptococcus_sp._'group_G' Streptococcus sp. 'group G'] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KBT FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kbt OCA], [https://pdbe.org/2kbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kbt RCSB], [https://www.ebi.ac.uk/pdbsum/2kbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kbt ProSAT]</span></td></tr>
{{STRUCTURE_2kbt|  PDB=2kbt  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.[https://www.uniprot.org/uniprot/VAV_MOUSE VAV_MOUSE] Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kbt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kbt ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sample solubility is essential for structural studies of proteins by solution NMR. Attachment of a solubility enhancement tag, such as GB1, MBP and thioredoxin, to a target protein has been used for this purpose. However, signal overlap of the tag with the target protein often made the spectral analysis difficult. Here we report a sortase-mediated protein ligation method to eliminate NMR signals arising from the tag by preparing the isotopically labeled target protein attached with the non-labeled GB1 tag at the C-terminus.


===Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method===
Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method.,Kobashigawa Y, Kumeta H, Ogura K, Inagaki F J Biomol NMR. 2009 Jan 13. PMID:19140010<ref>PMID:19140010</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_19140010}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2kbt" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19140010 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19140010}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2KBT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus,_synthetic,_streptococcus_sp._'group_g' Mus musculus, synthetic, streptococcus sp. 'group g']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBT OCA].
[[Category: Mus musculus]]
 
[[Category: Streptococcus sp. 'group G']]
==Reference==
[[Category: Synthetic construct]]
<ref group="xtra">PMID:19140010</ref><references group="xtra"/>
[[Category: Inagaki F]]
[[Category: Mus musculus, synthetic, streptococcus sp. 'group g']]
[[Category: Kobashigawa Y]]
[[Category: Inagaki, F.]]
[[Category: Kumeta H]]
[[Category: Kobashigawa, Y.]]
[[Category: Ogura K]]
[[Category: Kumeta, H.]]
[[Category: Ogura, K.]]
[[Category: Gb1]]
[[Category: Inset]]
[[Category: Intein]]
[[Category: Protein ligation]]
[[Category: Signaling protein]]
[[Category: Solubility enhancement]]
[[Category: Sortase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr  8 20:12:41 2009''

Latest revision as of 05:36, 15 May 2024

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

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