3gmb: Difference between revisions

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{{Seed}}
[[Image:3gmb.jpg|left|200px]]


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==Crystal Structure of 2-Methyl-3-hydroxypyridine-5-carboxylic acid Oxygenase==
The line below this paragraph, containing "STRUCTURE_3gmb", creates the "Structure Box" on the page.
<StructureSection load='3gmb' size='340' side='right'caption='[[3gmb]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3gmb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GMB FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_3gmb|  PDB=3gmb  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gmb OCA], [https://pdbe.org/3gmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gmb RCSB], [https://www.ebi.ac.uk/pdbsum/3gmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gmb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q988D3_RHILO Q988D3_RHILO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/3gmb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gmb ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A vitamin B(6) degradative pathway has recently been identified and characterized in Mesorhizobium loti MAFF303099. One of the enzymes on this pathway, 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO), is a flavin-dependent enzyme and catalyzes the oxidative ring-opening of 2-methyl-3-hydroxypyridine-5-carboxylic acid to form E-2-(acetamino-methylene)succinate. The gene for this enzyme has been cloned, and the corresponding protein has been overexpressed in Escherichia coli and purified. The crystal structure of MHPCO has been solved to 2.1 A using SAD phasing with and without the substrate MHPC bound. These crystal structures provide insight into the reaction mechanism and suggest roles for active site residues in the catalysis of a novel oxidative ring-opening reaction.


===Crystal Structure of 2-Methyl-3-hydroxypyridine-5-carboxylic acid Oxygenase===
Structure of the PLP Degradative Enzyme 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase from Mesorhizobium loti MAFF303099 and Its Mechanistic Implications.,McCulloch KM, Mukherjee T, Begley TP, Ealick SE Biochemistry. 2009 Apr 1. PMID:19317437<ref>PMID:19317437</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_19317437}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3gmb" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19317437 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19317437}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3GMB is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GMB OCA].
 
==Reference==
<ref group="xtra">PMID:19317437</ref><references group="xtra"/>
[[Category: Mesorhizobium loti]]
[[Category: Mesorhizobium loti]]
[[Category: Begley, T P.]]
[[Category: Begley TP]]
[[Category: Ealick, S E.]]
[[Category: Ealick SE]]
[[Category: McCulloch, K M.]]
[[Category: McCulloch KM]]
[[Category: Mukherjee, T.]]
[[Category: Mukherjee T]]
[[Category: Flavin monooxygenase]]
[[Category: Oxidoreductase]]
 
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Retrieved from "https://proteopedia.org/3gmb"