2rqe: Difference between revisions
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New page: '''Unreleased structure''' The entry 2rqe is ON HOLD Authors: Ochiai, M., Horiuchi, M., Kumeta, H., Ogura, K., Ashida, M., Inagaki, F. Description: Solution structure of the silkworm b... |
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The | ==Solution structure of the silkworm bGRP/GNBP3 N-terminal domain reveals the mechanism for b-1,3-glucan specific recognition== | ||
<StructureSection load='2rqe' size='340' side='right'caption='[[2rqe]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2rqe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqe OCA], [https://pdbe.org/2rqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqe RCSB], [https://www.ebi.ac.uk/pdbsum/2rqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqe ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/BGBP_BOMMO BGBP_BOMMO] Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.<ref>PMID:10671539</ref> <ref>PMID:3136171</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rq/2rqe_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rqe ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The beta-1,3-glucan recognition protein (betaGRP)/Gram-negative bacteria-binding protein 3 (GNBP3) is a crucial pattern-recognition receptor that specifically binds beta-1,3-glucan, a component of fungal cell walls. It evokes innate immunity against fungi through activation of the prophenoloxidase (proPO) cascade and Toll pathway in invertebrates. The betaGRP consists of an N-terminal beta-1,3-glucan-recognition domain and a C-terminal glucanase-like domain, with the former reported to be responsible for the proPO cascade activation. This report shows the solution structure of the N-terminal beta-1,3-glucan recognition domain of silkworm betaGRP. Although the N-terminal domain of betaGRP has a beta-sandwich fold, often seen in carbohydrate-binding modules, both NMR titration experiments and mutational analysis showed that betaGRP has a binding mechanism which is distinct from those observed in previously reported carbohydarate-binding domains. Our results suggest that betaGRP is a beta-1,3-glucan-recognition protein that specifically recognizes a triple-helical structure of beta-1,3-glucan. | |||
Solution structure of the silkworm betaGRP/GNBP3 N-terminal domain reveals the mechanism for beta-1,3-glucan-specific recognition.,Takahasi K, Ochiai M, Horiuchi M, Kumeta H, Ogura K, Ashida M, Inagaki F Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11679-84. Epub 2009 Jun 26. PMID:19561300<ref>PMID:19561300</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2rqe" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bombyx mori]] | |||
[[Category: Large Structures]] | |||
[[Category: Ashida M]] | |||
[[Category: Horiuchi M]] | |||
[[Category: Inagaki F]] | |||
[[Category: Kumeta H]] | |||
[[Category: Ochiai M]] | |||
[[Category: Ogura K]] | |||
[[Category: Takahasi K]] | |||
