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New page: left|200px<br /> <applet load="2v9v" size="450" color="white" frame="true" align="right" spinBox="true" caption="2v9v, resolution 1.10Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:2v9v.gif|left|200px]]<br />
<applet load="2v9v" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2v9v, resolution 1.10&Aring;" />
'''CRYSTAL STRUCTURE OF MOORELLA THERMOACETICA SELB(377-511)'''<br />


==Overview==
==Crystal Structure of Moorella thermoacetica SelB(377-511)==
The crystal structure of the first two winged-helix motifs of translation, elongation factor SelB from Moorella thermoacetica has been determined at, 1.1 A resolution. Compared with the previous structure of the two domains, in conjunction with winged-helix modules 3 and 4, the first winged-helix, domain underwent a substantial conformational change during which the, alpha-helical and beta-sheet portions of the element opened up like a, shell. This conformational rearrangement was elicited by a change in the, orientation of Trp396, leading to the disclosure of a bona fide, ligand-binding site in the direct vicinity of Trp396. Additionally, the, C-terminal tail of the second domain followed a different path compared, with the previous structure. It is conceivable that these conformational, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17881825 (full description)]]
<StructureSection load='2v9v' size='340' side='right'caption='[[2v9v]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2v9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V9V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9v OCA], [https://pdbe.org/2v9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v9v RCSB], [https://www.ebi.ac.uk/pdbsum/2v9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v9v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SELB_MOOTH SELB_MOOTH] Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/2v9v_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v9v ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the first two winged-helix motifs of translation elongation factor SelB from Moorella thermoacetica has been determined at 1.1 A resolution. Compared with the previous structure of the two domains in conjunction with winged-helix modules 3 and 4, the first winged-helix domain underwent a substantial conformational change during which the alpha-helical and beta-sheet portions of the element opened up like a shell. This conformational rearrangement was elicited by a change in the orientation of Trp396, leading to the disclosure of a bona fide ligand-binding site in the direct vicinity of Trp396. Additionally, the C-terminal tail of the second domain followed a different path compared with the previous structure. It is conceivable that these conformational switches constitute part of the molecular mechanism that underlies the communication between the N-terminal part of SelB, which binds Sec-tRNA(Sec) and GTP, and the C-terminal part of the protein, which binds selenocysteine-insertion sequences.


==About this Structure==
Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB.,Ganichkin O, Wahl MC Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:17881825<ref>PMID:17881825</ref>
2V9V is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]] with CL and NA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V9V OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Conformational switches in winged-helix domains 1 and 2 of bacterial translation elongation factor SelB., Ganichkin O, Wahl MC, Acta Crystallogr D Biol Crystallogr. 2007 Oct;63(Pt 10):1075-81. Epub 2007, Sep 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17881825 17881825]
</div>
<div class="pdbe-citations 2v9v" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
*[[SelB|SelB]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Moorella thermoacetica]]
[[Category: Moorella thermoacetica]]
[[Category: Single protein]]
[[Category: Ganichkin O]]
[[Category: Ganichkin, O.]]
[[Category: Wahl MC]]
[[Category: Wahl, M.C.]]
[[Category: CL]]
[[Category: NA]]
[[Category: cytoplasm]]
[[Category: gtp-binding]]
[[Category: nucleotide-binding]]
[[Category: protein biosynthesis]]
[[Category: protein conformational change]]
[[Category: protein dynamics]]
[[Category: selenocysteine]]
[[Category: selenoprotein biosynthesis]]
[[Category: transcription elongation factor selb]]
[[Category: winged-helix domain]]
 
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