2que: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,327,076 edits
New page: left|200px<br /><applet load="2que" size="450" color="white" frame="true" align="right" spinBox="true" caption="2que, resolution 2.25Å" /> '''Saturation of substr...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,327,076 edits
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2que.jpg|left|200px]]<br /><applet load="2que" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2que, resolution 2.25&Aring;" />
'''Saturation of substrate-binding site using two natural ligands: Crystal structure of a ternary complex of phospholipase A2 with anisic acid and ajmaline at 2.25 A resolution'''<br />


==About this Structure==
==Saturation of substrate-binding site using two natural ligands: Crystal structure of a ternary complex of phospholipase A2 with anisic acid and ajmaline at 2.25 A resolution==
2QUE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Daboia_russellii_pulchella Daboia russellii pulchella] with AJM and ANN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2QUE OCA].
<StructureSection load='2que' size='340' side='right'caption='[[2que]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
[[Category: Daboia russellii pulchella]]
== Structural highlights ==
[[Category: Phospholipase A(2)]]
<table><tr><td colspan='2'>[[2que]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Daboia_russelii_pulchella Daboia russelii pulchella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QUE FirstGlance]. <br>
[[Category: Single protein]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
[[Category: Kaur, P.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AJM:AJMALINE'>AJM</scene>, <scene name='pdbligand=ANN:4-METHOXYBENZOIC+ACID'>ANN</scene></td></tr>
[[Category: Kumar, S.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2que FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2que OCA], [https://pdbe.org/2que PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2que RCSB], [https://www.ebi.ac.uk/pdbsum/2que PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2que ProSAT]</span></td></tr>
[[Category: Sharma, S.]]
</table>
[[Category: Singh, N.]]
== Function ==
[[Category: Singh, T.P.]]
[https://www.uniprot.org/uniprot/PA2B8_DABRR PA2B8_DABRR] Snake venom phospholipase A2 (PLA2) that shows weak neurotoxicity and medium anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC(50) is 130 nM) (PubMed:18062812). It also damages vital organs such as lung, liver and kidney, displays edema-inducing activities when injected into the foot pads of mice and induces necrosis of muscle cells when injected into the thigh muscle. Has a low enzymatic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:18062812</ref> <ref>PMID:2115497</ref> <ref>PMID:8835338</ref>
[[Category: AJM]]
== Evolutionary Conservation ==
[[Category: ANN]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: active site]]
Check<jmol>
[[Category: binding affinity]]
  <jmolCheckbox>
[[Category: calcium]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qu/2que_consurf.spt"</scriptWhenChecked>
[[Category: hydrolase]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
[[Category: lipid degradation]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: lipid hydrolysis]]
  </jmolCheckbox>
[[Category: metal-binding]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2que ConSurf].
[[Category: neurotoxin]]
<div style="clear:both"></div>
[[Category: presynaptic neurotoxin]]
[[Category: secreted]]
[[Category: toxin]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:55:15 2007''
==See Also==
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Daboia russelii pulchella]]
[[Category: Large Structures]]
[[Category: Kaur P]]
[[Category: Kumar S]]
[[Category: Sharma S]]
[[Category: Singh N]]
[[Category: Singh TP]]
Retrieved from "https://proteopedia.org/2que"