2v6e: Difference between revisions

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New page: left|200px<br /> <applet load="2v6e" size="450" color="white" frame="true" align="right" spinBox="true" caption="2v6e, resolution 3.2Å" /> '''PROTELOMERASE TELK C...
 
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[[Image:2v6e.gif|left|200px]]<br />
<applet load="2v6e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2v6e, resolution 3.2&Aring;" />
'''PROTELOMERASE TELK COMPLEXED WITH SUBSTRATE DNA'''<br />


==Overview==
==protelomerase TelK complexed with substrate DNA==
The termini of linear chromosomes are protected by specialized DNA, structures known as telomeres that also facilitate the complete, replication of DNA ends. The simplest type of telomere is a covalently, closed DNA hairpin structure found in linear chromosomes of prokaryotes, and viruses. Bidirectional replication of a chromosome with hairpin, telomeres produces a catenated circular dimer that is subsequently, resolved into unit-length chromosomes by a dedicated DNA, cleavage-rejoining enzyme known as a hairpin telomere resolvase, (protelomerase). Here we report a crystal structure of the protelomerase, TelK from Klebsiella oxytoca phage varphiKO2, in complex with the, palindromic target DNA. The structure shows the TelK dimer destabilizes, base pairing interactions to promote the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17889664 (full description)]]
<StructureSection load='2v6e' size='340' side='right'caption='[[2v6e]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2v6e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N15 Escherichia virus N15] and [https://en.wikipedia.org/wiki/Klebsiella_phage_phiKO2 Klebsiella phage phiKO2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V6E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v6e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v6e OCA], [https://pdbe.org/2v6e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v6e RCSB], [https://www.ebi.ac.uk/pdbsum/2v6e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v6e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PTELO_BPKO2 PTELO_BPKO2] Converts the circular intermediates produced by the viral replication and carrying a joined telomere site to a linear DNA molecule with covalently closed hairpin ends (PubMed:14996813). The viral circular DNA is cleaved at a palindromic site called telRL thereby generating a linear prophage plasmid with telomeres (PubMed:14996813). Binds covalently to the 3'-phosphoryl of the cleaved strands (By similarity).[UniProtKB:Q77WP1]<ref>PMID:14996813</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The termini of linear chromosomes are protected by specialized DNA structures known as telomeres that also facilitate the complete replication of DNA ends. The simplest type of telomere is a covalently closed DNA hairpin structure found in linear chromosomes of prokaryotes and viruses. Bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated DNA cleavage-rejoining enzyme known as a hairpin telomere resolvase (protelomerase). Here we report a crystal structure of the protelomerase TelK from Klebsiella oxytoca phage varphiKO2, in complex with the palindromic target DNA. The structure shows the TelK dimer destabilizes base pairing interactions to promote the refolding of cleaved DNA ends into two hairpin ends. We propose that the hairpinning reaction is made effectively irreversible by a unique protein-induced distortion of the DNA substrate that prevents religation of the cleaved DNA substrate.


==About this Structure==
An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres.,Aihara H, Huang WM, Ellenberger T Mol Cell. 2007 Sep 21;27(6):901-13. PMID:17889664<ref>PMID:17889664</ref>
2V6E is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Klebsiella_phage_phiko2 Klebsiella phage phiko2]] with VO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V6E OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
An Interlocked Dimer of the Protelomerase TelK Distorts DNA Structure for the Formation of Hairpin Telomeres., Aihara H, Huang WM, Ellenberger T, Mol Cell. 2007 Sep 21;27(6):901-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17889664 17889664]
</div>
[[Category: Klebsiella phage phiko2]]
<div class="pdbe-citations 2v6e" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Aihara, H.]]
<references/>
[[Category: Ellenberger, T.]]
__TOC__
[[Category: Huang, W.M.]]
</StructureSection>
[[Category: VO4]]
[[Category: Escherichia virus N15]]
[[Category: dna distortion]]
[[Category: Klebsiella phage phiKO2]]
[[Category: hairpin telomere]]
[[Category: Large Structures]]
[[Category: hydrolase]]
[[Category: Aihara H]]
[[Category: protelomerase]]
[[Category: Ellenberger T]]
[[Category: resolvase]]
[[Category: Huang WM]]
 
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