1e4p: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1e4p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e4p" /> '''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN ... |
No edit summary |
||
| (15 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | ==Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site== | ||
The cleavage site of the Neurospora VS RNA ribozyme is located in a | <StructureSection load='1e4p' size='340' side='right'caption='[[1e4p]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1e4p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora Neurospora]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E4P FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e4p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e4p OCA], [https://pdbe.org/1e4p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e4p RCSB], [https://www.ebi.ac.uk/pdbsum/1e4p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e4p ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure. | |||
Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site.,Michiels PJ, Schouten CH, Hilbers CW, Heus HA RNA. 2000 Dec;6(12):1821-32. PMID:11142381<ref>PMID:11142381</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1e4p" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribozyme 3D structures|Ribozyme 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Neurospora]] | |||
[[Category: Heus HA]] | |||
[[Category: Hilbers CW]] | |||
[[Category: Michiels PJA]] | |||
[[Category: Schouten CHJ]] | |||
Latest revision as of 05:29, 15 May 2024
Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site
| ||||||||||||
