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New page: left|200px<br /><applet load="1qza" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qza" /> '''Coordinates of the A/T site tRNA model fitte...
 
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[[Image:1qza.gif|left|200px]]<br /><applet load="1qza" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1qza" />
'''Coordinates of the A/T site tRNA model fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome'''<br />


==Overview==
==Coordinates of the A/T site tRNA model fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome==
Aminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the, ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we, present a cryo-electron microscopy (cryo-EM) study, at a resolution of, approximately 9 A, showing that during the incorporation of the aa-tRNA, into the 70S ribosome of Escherichia coli, the flexibility of aa-tRNA, allows the initial codon recognition and its accommodation into the, ribosomal A site. In addition, a conformational change observed in the, GTPase-associated center (GAC) of the ribosomal 50S subunit may provide, the mechanism by which the ribosome promotes a relative movement of the, aa-tRNA with respect to EF-Tu. This relative rearrangement seems to, facilitate codon recognition by the incoming aa-tRNA, and to provide the, codon-anticodon recognition-dependent signal for the GTPase activity of, EF-Tu. From these new findings we propose a mechanism that can explain the, sequence of events during the decoding of mRNA on the ribosome.
<SX load='1qza' size='340' side='right' viewer='molstar' caption='[[1qza]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1qza]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZA FirstGlance]. <br>
1QZA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QZA OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qza OCA], [https://pdbe.org/1qza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qza RCSB], [https://www.ebi.ac.uk/pdbsum/1qza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qza ProSAT]</span></td></tr>
==Reference==
</table>
Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy., Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J, Nat Struct Biol. 2003 Nov;10(11):899-906. Epub 2003 Oct 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14566331 14566331]
__TOC__
[[Category: Protein complex]]
</SX>
[[Category: Ehrenberg, M.]]
[[Category: Escherichia coli]]
[[Category: Frank, J.]]
[[Category: Large Structures]]
[[Category: Harvey, S.C.]]
[[Category: Ehrenberg M]]
[[Category: Li, W.]]
[[Category: Frank J]]
[[Category: Nielsen, R.C.]]
[[Category: Harvey SC]]
[[Category: Nissen, P.]]
[[Category: Li W]]
[[Category: Sengupta, J.]]
[[Category: Nielsen RC]]
[[Category: Stagg, S.M.]]
[[Category: Nissen P]]
[[Category: Valle, M.]]
[[Category: Sengupta J]]
[[Category: Zavialov, A.]]
[[Category: Stagg SM]]
[[Category: a/t-site trna.]]
[[Category: Valle M]]
[[Category: decoding]]
[[Category: Zavialov A]]
[[Category: trna model]]
 
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