1idw: Difference between revisions
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New page: left|200px<br /><applet load="1idw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1idw, resolution 1.8Å" /> '''STRUCTURE OF THE HYBR... |
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== | ==STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[CL]U IN PRESENCE OF RH(NH3)6+++== | ||
In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA | <StructureSection load='1idw' size='340' side='right'caption='[[1idw]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1idw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RHD:RHODIUM+HEXAMINE+ION'>RHD</scene>, <scene name='pdbligand=UCL:5-CHLORO-2-DEOXYURIDINE+5-(DIHYDROGEN+PHOSPHATE)'>UCL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1idw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idw OCA], [https://pdbe.org/1idw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1idw RCSB], [https://www.ebi.ac.uk/pdbsum/1idw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1idw ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In the presence of cobalt, rhodium or iridium hexammine salts, the RNA/DNA hybrid r-GCUUCGGC-d(X)U (with X = F, Cl or Br) crystallizes as a double-stranded helix with four consecutive G-U and C-U mismatches. The deoxy chloro- and bromouracil derivatives are isomorphous, space group C2, unit-cell parameters a = 53.80, b = 19.40, c = 50.31 A, beta = 109.9 degrees, with the same infinite helix arrangement in the packing along the c axis with one extra DNA halogenouracil base included in the stacking. However, the fluorouracil derivative, with unit-cell parameters a = 53.75, b = 19.40, c = 45.84 A, beta = 105.7 degrees, is not isomorphous. The corresponding extra DNA base d(F)U of the second strand is ejected out of the helical stack, leading to a shortening of the c axis. The specific destabilization of the fluorouracil for the duplex building is analyzed in terms of the polarization effect of the halogen atom attached to the 3'-terminal base that modulates its interactions. | |||
Destabilizing effect of a fluorouracil extra base in a hybrid RNA duplex compared with bromo and chloro analogues.,Cruse W, Saludjian P, Neuman A, Prange T Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1609-13. Epub 2001, Oct 25. PMID:11679725<ref>PMID:11679725</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1idw" style="background-color:#fffaf0;"></div> | ||
[[Category: Cruse | == References == | ||
[[Category: Neuman | <references/> | ||
[[Category: Prange | __TOC__ | ||
[[Category: Saludjian | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Cruse W]] | |||
[[Category: Neuman A]] | |||
[[Category: Prange T]] | |||
[[Category: Saludjian P]] | |||
Latest revision as of 11:22, 2 August 2023
STRUCTURE OF THE HYBRID RNA/DNA R-GCUUCGGC-D[CL]U IN PRESENCE OF RH(NH3)6+++
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