1qzn: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
New page: left|200px<br /><applet load="1qzn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qzn, resolution 1.90Å" /> '''Crystal Structure An...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(13 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1qzn.jpg|left|200px]]<br /><applet load="1qzn" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1qzn, resolution 1.90&Aring;" />
'''Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus'''<br />


==Overview==
==Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus==
The incorporation of enzymes into the multi-enzyme cellulosome complex and, its anchoring to the bacterial cell surface are dictated by a set of, binding interactions between two complementary protein modules: the, cohesin and the dockerin. In this work, the X-ray crystal structure of a, type-II cohesin from scaffoldin A of Bacteroides cellulosolvens has been, determined to a resolution of 1.6 angstroms using molecular replacement., The type-II B. cellulosolvens cohesin (Bc-cohesin-II) is the first, detailed description of a crystal structure for a type-II cohesin, and its, features were compared with the known type-I cohesins from Clostridium, thermocellum and Clostridium cellulolyticum (Ct-cohesin-I and, Cc-cohesin-I, respectively). The overall jelly-roll topology of the, type-II Bc-cohesin is very similar to that observed for the type-I, cohesins with three additional secondary structures: an alpha-helix and, two "beta-flaps" that disrupt the normal course of a beta-strand. In, addition, beta-strand 5 is elevated by approximately 4 angstroms on the, surface of the molecule, relative to the type-I Ct and Cc-cohesins. Like, its type-I analogue, the hydrophobic/aromatic core of Bc-cohesin-II, comprises an upper and lower core, but an additional aromatic patch and, conserved tryptophan at the crown of the molecule serves to stabilize the, alpha-helix of the type-II cohesin. Comparison of Bc-cohesin-II with the, known type-I cohesin-dockerin heterodimer suggests that each of the, additional secondary structural elements assumes a flanking position, relative to the putative dockerin-binding surface. The raised ridge formed, by beta-strand 5 confers additional distinctive topographic features to, the proposed binding interface that collectively distinguish between the, type-II and type-I cohesins.
<StructureSection load='1qzn' size='340' side='right'caption='[[1qzn]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1qzn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_cellulolyticus Acetivibrio cellulolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZN FirstGlance]. <br>
1QZN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acetivibrio_cellulolyticus Acetivibrio cellulolyticus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QZN OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzn OCA], [https://pdbe.org/1qzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzn RCSB], [https://www.ebi.ac.uk/pdbsum/1qzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzn ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements., Noach I, Frolow F, Jakoby H, Rosenheck S, Shimon LW, Lamed R, Bayer EA, J Mol Biol. 2005 Apr 22;348(1):1-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15808849 15808849]
== Function ==
[https://www.uniprot.org/uniprot/Q7WYN3_9FIRM Q7WYN3_9FIRM]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzn ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Acetivibrio cellulolyticus]]
[[Category: Acetivibrio cellulolyticus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bayer, E.A.]]
[[Category: Bayer EA]]
[[Category: Frolow, F.]]
[[Category: Frolow F]]
[[Category: Lamed, R.]]
[[Category: Lamed R]]
[[Category: Noach, I.]]
[[Category: Noach I]]
[[Category: Qi, X.]]
[[Category: Qi X]]
[[Category: Rosenheck, S.]]
[[Category: Rosenheck S]]
[[Category: Shimon, L.J.W.]]
[[Category: Shimon LJW]]
[[Category: keywords: cohesins type ii; cellulosome;]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 21:51:41 2007''

Latest revision as of 08:19, 14 February 2024

Crystal Structure Analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus

1qzn, resolution 1.90Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1qzn&oldid=4054070"