3d4x: Difference between revisions

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{{Seed}}
[[Image:3d4x.jpg|left|200px]]


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==Crystal structure determination of cat (Felis silvestris catus) hemoglobin at 2.2 angstrom resolution==
The line below this paragraph, containing "STRUCTURE_3d4x", creates the "Structure Box" on the page.
<StructureSection load='3d4x' size='340' side='right'caption='[[3d4x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3d4x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Felis_catus Felis catus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D4X FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
{{STRUCTURE_3d4x|  PDB=3d4x  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d4x OCA], [https://pdbe.org/3d4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d4x RCSB], [https://www.ebi.ac.uk/pdbsum/3d4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d4x ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HBA_FELCA HBA_FELCA] Involved in oxygen transport from the lung to the various peripheral tissues.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d4/3d4x_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d4x ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Haemoglobin is a tetrameric protein that plays a vital role in the transport of oxygen from the lungs to the tissues and of carbon dioxide back to the lungs. Even though a large amount of work has already been performed in this area, the study of the haemoglobin structures of avian and mammalian species is rather incomplete. Efforts are being made to understand the salient features of the species mentioned above. Here, whole blood plasma was collected from sheep and goat and purified by anion-exchange chromatography; the haemoglobins were crystallized by the hanging-drop vapour-diffusion method under unbuffered low-salt conditions using PEG 3350 as a precipitant. Data collection was carried out using a MAR345 image-plate detector system. Sheep haemoglobin crystallizes in the orthorhombic space group P2(1)2(1)2(1) with one whole biological molecule (alpha2beta2) in the asymmetric unit, with unit-cell parameters a = 60.231, b = 70.695, c = 131.479 A. In contrast, goat haemoglobin crystallizes in the triclinic system with two biological molecules (alpha2beta2) in the unit cell. The unit-cell parameters are a = 53.103, b = 69.382, c = 96.098 A, alpha = 110.867, beta = 91.133, gamma = 109.437 degrees.


===Crystal structure determination of cat (Felis silvestris catus) hemoglobin at 2.2 angstrom resolution===
Crystallization of sheep (Ovis aries) and goat (Capra hircus) haemoglobins under unbuffered low-salt conditions.,Neelagandan K, Moorthy PS, Balasubramanian M, Ponnuswamy MN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt, 10):887-9. Epub 2007 Sep 19. PMID:017909297<ref>PMID:017909297</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3d4x" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
3D4X is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Felis_catus Felis catus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D4X OCA].
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:17909297</ref><ref group="xtra">PMID:19255493</ref><ref group="xtra">PMID:19200047</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Felis catus]]
[[Category: Felis catus]]
[[Category: Balasubramanian, M.]]
[[Category: Large Structures]]
[[Category: Moorthy, Pon Sathya.]]
[[Category: Balasubramanian M]]
[[Category: Neelagandan, K.]]
[[Category: Neelagandan K]]
[[Category: Ponnuswamy, M N.]]
[[Category: Ponnuswamy MN]]
[[Category: Allosteric mechanism]]
[[Category: Sathya Moorthy P]]
[[Category: Felis silvestris catus]]
[[Category: Heme]]
[[Category: Hemoglobin]]
[[Category: Iron]]
[[Category: Low oxygen affinity]]
[[Category: Monoclinic]]
[[Category: Oxygen storage]]
[[Category: Oxygen transport]]
[[Category: Polymorphism]]
[[Category: Transport]]
 
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Retrieved from "https://proteopedia.org/3d4x"