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New page: left|200px<br /><applet load="1edr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1edr, resolution 1.60Å" /> '''MOLECULAR AND CRYSTA...
 
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[[Image:1edr.gif|left|200px]]<br /><applet load="1edr" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1edr, resolution 1.60&Aring;" />
'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG) AT 1.6 ANGSTROM'''<br />


==Overview==
==MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG) AT 1.6 ANGSTROM==
In a previous paper, 2'-deoxy-N(6)-methoxyadenosine (mo(6)A) was shown to, form a mismatch base-pair with 2'-deoxycytidine with a Watson-Crick-type, geometry. To fully understand the structural basis of genetic mutations, with damaged DNA, it is necessary to examine whether the methoxylated, adenine residue still has the ability to form the regular Watson-Crick, pairing with a thymine residue. Therefore, a DNA dodecamer with the, sequence d(CGCGmo(6)AATTCGCG) has been synthesized and its crystal, structure determined. The methoxylation has no significant effect on the, overall DNA conformation, which is that of a standard B-form duplex. The, methoxylated adenine moieties adopt the amino tautomer with an anti, conformation around the C(6)-N(6) bond to the N(1) atom, and they form a, Watson-Crick base-pair with thymine residues on the opposite strand, similar to an unmodified adenine residue. It is concluded that, methoxylated adenine can present two alternate faces for base-pairing, thanks to the amino&lt;--&gt;imino tautomerism allowed by methoxylation. Based, on this property, two gene transition routes are proposed.
<StructureSection load='1edr' size='340' side='right'caption='[[1edr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1edr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EDR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EDR FirstGlance]. <br>
1EDR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EDR OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A47:N6-METHOXY+ADENOSINE+5-MONOPHOSPHATE'>A47</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1edr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1edr OCA], [https://pdbe.org/1edr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1edr RCSB], [https://www.ebi.ac.uk/pdbsum/1edr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1edr ProSAT]</span></td></tr>
Crystallographic studies on damaged DNAs. II. N(6)-methoxyadenine can present two alternate faces for Watson-Crick base-pairing, leading to pyrimidine transition mutagenesis., Chatake T, Hikima T, Ono A, Ueno Y, Matsuda A, Takenaka A, J Mol Biol. 1999 Dec 17;294(5):1223-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600380 10600380]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Chatake, T.]]
</StructureSection>
[[Category: Hikima, T.]]
[[Category: Large Structures]]
[[Category: Matsuda, A.]]
[[Category: Chatake T]]
[[Category: Ono, A.]]
[[Category: Hikima T]]
[[Category: Takenaka, A.]]
[[Category: Matsuda A]]
[[Category: Ueno, Y.]]
[[Category: Ono A]]
[[Category: MG]]
[[Category: Takenaka A]]
[[Category: SPM]]
[[Category: Ueno Y]]
[[Category: b-dna]]
[[Category: damaged dna]]
[[Category: deoxyribonucleic acid]]
[[Category: double helix]]
[[Category: methoxyadenosine]]
[[Category: modified nucleotide]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:05:27 2007''

Latest revision as of 07:01, 7 February 2024

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG) AT 1.6 ANGSTROM

1edr, resolution 1.60Å

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