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New page: left|200px<br /><applet load="178d" size="450" color="white" frame="true" align="right" spinBox="true" caption="178d, resolution 2.500Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:178d.gif|left|200px]]<br /><applet load="178d" size="450" color="white" frame="true" align="right" spinBox="true"
caption="178d, resolution 2.500&Aring;" />
'''CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS==
The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing, the chemically modified base 8-hydroxydeoxyguanine (O8G), has been, determined at 2.5-A resolution and refined to a crystallographic R-factor, of 16.8%. The B-type DNA helix contains standard Watson-Crick base pairs, except at the mismatch sites, where O8G adopts a syn conformation and, forms hydrogen bonds to adenine in the anti conformation. The, thermodynamic stability of the duplex was found by UV melting techniques, to be intermediate between the native oligonucleotide d(CGCAAATTTGCG) and, an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG). Comparison of, the structure of the O8G(syn).A(anti) base pair with those of Watson-Crick, base pairs has given a reason why O8G.A base pairs are not well repaired, by DNA proofreading enzymes.
<StructureSection load='178d' size='340' side='right'caption='[[178d]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[178d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=178D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=178D FirstGlance]. <br>
178D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=178D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=178d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=178d OCA], [https://pdbe.org/178d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=178d RCSB], [https://www.ebi.ac.uk/pdbsum/178d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=178d ProSAT]</span></td></tr>
Crystal structure of a DNA duplex containing 8-hydroxydeoxyguanine-adenine base pairs., McAuley-Hecht KE, Leonard GA, Gibson NJ, Thomson JB, Watson WP, Hunter WN, Brown T, Biochemistry. 1994 Aug 30;33(34):10266-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8068665 8068665]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Brown, T.]]
</StructureSection>
[[Category: Gibson, N.J.]]
[[Category: Large Structures]]
[[Category: Hunter, W.N.]]
[[Category: Brown T]]
[[Category: Leonard, G.A.]]
[[Category: Gibson NJ]]
[[Category: McAuley-Hecht, K.E.]]
[[Category: Hunter WN]]
[[Category: Thomson, J.B.]]
[[Category: Leonard GA]]
[[Category: Watson, W.P.]]
[[Category: McAuley-Hecht KE]]
[[Category: b-dna]]
[[Category: Thomson JB]]
[[Category: double helix]]
[[Category: Watson WP]]
[[Category: mismatched]]
[[Category: modified]]
 
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