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New page: left|200px<br /><applet load="1eg6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eg6, resolution 2.00Å" /> '''CRYSTAL STRUCTURE AN...
 
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[[Image:1eg6.gif|left|200px]]<br /><applet load="1eg6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1eg6, resolution 2.00&Aring;" />
'''CRYSTAL STRUCTURE ANALYSIS OF D(CG(5-BRU)ACG) COMPLEXES TO A PHENAZINE'''<br />


==Overview==
==CRYSTAL STRUCTURE ANALYSIS OF D(CG(5-BRU)ACG) COMPLEXES TO A PHENAZINE==
The structure of the duplex d[CG(5-BrU)ACG](2) bound to, 9-bromophenazine-4-carboxamide has been solved through MAD phasing at 2.0, A resolution. It shows an unexpected and previously unreported, intercalation cavity stabilized by the drug and novel binding modes of, Co(2+) ions at certain guanine N7 sites. For the intercalation cavity the, terminal cytosine is rotated to pair with the guanine of a, symmetry-related duplex to create a pseudo-Holliday junction geometry, with two such cavities linked through the minor groove interactions of the, N2/N3 guanine sites at an angle of 40 degrees, creating a quadruplex-like, structure. The mode of binding of the drug is shown to be disordered, with, the major conformations showing the side chain bound to the N7 position of, adjacent guanines. The other end of the duplex exhibits a terminal base, fraying in the presence of Co(2+) ions linking symmetry-related guanines, causing the helices to intertwine through the minor groove. The, stabilization of the structure by the intercalating drug shows that this, class of compound may bind to DNA junctions as well as duplex DNA or to, strand-nicked DNA ('hemi-intercalated'), as in the cleavable complex. This, suggests a structural basis for the dual poisoning of topoisomerase I and, II enzymes by this family of drugs.
<StructureSection load='1eg6' size='340' side='right'caption='[[1eg6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1eg6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG6 FirstGlance]. <br>
1EG6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with 3CO, BR and BFA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EG6 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CO:COBALT+(III)+ION'>3CO</scene>, <scene name='pdbligand=BFA:9-BROMO-PHENAZINE-1-CARBOXYLIC+ACID+(2-DIMETHYLAMINO-ETHYL)-AMIDE'>BFA</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg6 OCA], [https://pdbe.org/1eg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg6 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg6 ProSAT]</span></td></tr>
Guanine specific binding at a DNA junction formed by d[CG(5-BrU)ACG](2) with a topoisomerase poison in the presence of Co(2+) ions., Thorpe JH, Hobbs JR, Todd AK, Denny WA, Charlton P, Cardin CJ, Biochemistry. 2000 Dec 12;39(49):15055-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11106483 11106483]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Cardin, C.J.]]
</StructureSection>
[[Category: Denny, W.A.]]
[[Category: Large Structures]]
[[Category: Hobbs, J.R.]]
[[Category: Cardin CJ]]
[[Category: Thorpe, J.H.]]
[[Category: Denny WA]]
[[Category: 3CO]]
[[Category: Hobbs JR]]
[[Category: BFA]]
[[Category: Thorpe JH]]
[[Category: BR]]
[[Category: double helix]]
[[Category: drug-dna complex]]
[[Category: intercalation]]
[[Category: major groove binding]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:12:10 2007''

Latest revision as of 07:02, 7 February 2024

CRYSTAL STRUCTURE ANALYSIS OF D(CG(5-BRU)ACG) COMPLEXES TO A PHENAZINE

1eg6, resolution 2.00Å

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