2kjo: Difference between revisions
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New page: '''Unreleased structure''' The entry 2kjo is ON HOLD Authors: georgescu, j., bechinger, b. Description: pH dependent structures of LAH4 in micellar environment: mode of acting ''Page ... |
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The | ==pH dependent structures of LAH4 in micellar environment: mode of acting== | ||
<StructureSection load='2kjo' size='340' side='right'caption='[[2kjo]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kjo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KJO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kjo OCA], [https://pdbe.org/2kjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kjo RCSB], [https://www.ebi.ac.uk/pdbsum/2kjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kjo ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The LAH4 family of histidine-rich peptides exhibits potent antimicrobial and DNA transfection activities, both of which require interactions with cellular membranes. The bilayer association of the peptides has been shown to be strongly pH-dependent, with in-planar alignments under acidic conditions and transmembrane orientations when the histidines are discharged. Therefore, we investigated the pH- and temperature-dependent conformations of LAH4 in DPC micellar solutions and in a TFE/PBS solvent mixture. In the presence of detergent and at pH 4.1, LAH4 adopts helical conformations between residues 9 and 24 concomitantly with a high hydrophobic moment. At pH 6.1, a helix-loop-helix structure forms with a hinge encompassing residues His(1)-Ala(1)(3). The data suggest that the high density of histidine residues and the resulting electrostatic repulsion lead to both a decrease in the pK values of the histidines and a less stable alpha-helical conformation of this region. The hinged structure at pH 6.1 facilitates membrane anchoring and insertion. At pH 7.8, the histidines are uncharged and an extended helical conformation including residues 4-21 is again obtained. LAH4 thus exhibits a high degree of conformational plasticity. The structures provide a stroboscopic view of the conformational changes that occur during membrane insertion, and are discussed in the context of antimicrobial activity and DNA transfection. | |||
NMR structures of the histidine-rich peptide LAH4 in micellar environments: membrane insertion, pH-dependent mode of antimicrobial action, and DNA transfection.,Georgescu J, Munhoz VH, Bechinger B Biophys J. 2010 Oct 20;99(8):2507-15. PMID:20959091<ref>PMID:20959091</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2kjo" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Bechinger B]] | |||
[[Category: Georgescu J]] | |||
Latest revision as of 09:48, 9 May 2024
pH dependent structures of LAH4 in micellar environment: mode of acting
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