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New page: left|200px<br /><applet load="1enn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1enn, resolution 0.89Å" /> '''SOLVENT ORGANIZATION...
 
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[[Image:1enn.gif|left|200px]]<br /><applet load="1enn" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1enn, resolution 0.89&Aring;" />
'''SOLVENT ORGANIZATION IN AN OLIGONUCLEOTIDE CRYSTAL: THE STRUCTURE OF D(GCGAATTCG)2 AT ATOMIC RESOLUTION'''<br />


==Overview==
==SOLVENT ORGANIZATION IN AN OLIGONUCLEOTIDE CRYSTAL: THE STRUCTURE OF D(GCGAATTCG)2 AT ATOMIC RESOLUTION==
We describe the crystal structure of d(GCGAATTCG) determined by x-ray, diffraction at atomic resolution level (0.89 A). The duplex structure is, practically identical to that described at 2.05 A resolution (Van, Meervelt, L., Vlieghe, D., Dautant, A., Gallois, B., Precigoux, G., and, Kennard, O. (1995) Nature 374, 742-744), however about half of the, phosphate groups show multiple conformations. The crystal has three, regions with different solvent structure. One of them contains several, ordered Mg(+2) ions and can be considered as an ionic crystal. A second, region is formed by a network of ordered water molecules with a polygonal, organization that binds three duplexes. The third region is formed by, channels of solvent in which very few ordered solvent molecules are, visible. The less ordered phosphates are found facing this channel. The, latter region provides a view of DNA with highly movable charges, both, negative phosphates and counterions, without a precise location.
<StructureSection load='1enn' size='340' side='right'caption='[[1enn]], [[Resolution|resolution]] 0.89&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1enn]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=475d 475d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ENN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ENN FirstGlance]. <br>
1ENN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, CL and O as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 475D. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ENN OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.89&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1enn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1enn OCA], [https://pdbe.org/1enn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1enn RCSB], [https://www.ebi.ac.uk/pdbsum/1enn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1enn ProSAT]</span></td></tr>
Solvent organization in an oligonucleotide crystal. The structure of d(GCGAATTCG)2 at atomic resolution., Soler-Lopez M, Malinina L, Subirana JA, J Biol Chem. 2000 Jul 28;275(30):23034-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10749874 10749874]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Malinina, L.]]
</StructureSection>
[[Category: Soler-Lopez, M.]]
[[Category: Large Structures]]
[[Category: Subirana, J.A.]]
[[Category: Malinina L]]
[[Category: CL]]
[[Category: Soler-Lopez M]]
[[Category: MG]]
[[Category: Subirana JA]]
[[Category: O]]
[[Category: atomic resolution]]
[[Category: b-dna]]
[[Category: base triplet]]
[[Category: hydration]]
[[Category: ions]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:34:39 2007''
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