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New page: left|200px<br /><applet load="1n5s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n5s, resolution 1.70Å" /> '''Crystal structure of...
 
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[[Image:1n5s.gif|left|200px]]<br /><applet load="1n5s" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1n5s, resolution 1.70&Aring;" />
'''Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Acetyl Dithranol'''<br />


==Overview==
==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Acetyl Dithranol==
ActVA-Orf6 monooxygenase from Streptomyces coelicolor that catalyses the, oxidation of an aromatic intermediate of the actinorhodin biosynthetic, pathway is a member of a class of small monooxygenases that carry out, oxygenation without the assistance of any of the prosthetic groups, metal, ions or cofactors normally associated with activation of molecular oxygen., The overall structure is a ferredoxin-like fold with a novel dimeric, assembly, indicating that the widely represented ferredoxin fold may, sustain yet another functionality. The resolution (1.3 A) of the enzyme, structure and its complex with substrate and product analogues allows us, to visualize the mechanism of binding and activation of the substrate for, attack by molecular oxygen, and utilization of two gates for the reaction, components including a proton gate and an O(2)/H(2)O gate with a putative, protein channel. This is the first crystal structure of an enzyme involved, in the tailoring of a type II aromatic polyketide and illustrates some of, the enzyme-substrate recognition features that may apply to a range of, other enzymes involved in modifying a polyketide core structure.
<StructureSection load='1n5s' size='340' side='right'caption='[[1n5s]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1n5s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N5S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PG:2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL'>1PG</scene>, <scene name='pdbligand=ADL:(1,8-DIHYDROXY-9-OXO-9,10-DIHYDRO-ANTHRACEN-2-YL)-ACETIC+ACID'>ADL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5s OCA], [https://pdbe.org/1n5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n5s RCSB], [https://www.ebi.ac.uk/pdbsum/1n5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q53908_STRCH Q53908_STRCH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/1n5s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n5s ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1N5S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor] with ADL and 1PG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N5S OCA].
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis., Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B, EMBO J. 2003 Jan 15;22(2):205-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12514126 12514126]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Federici L]]
[[Category: Streptomyces coelicolor]]
[[Category: Kendrew SG]]
[[Category: Federici, L.]]
[[Category: Malatesta F]]
[[Category: Kendrew, S.G.]]
[[Category: Marsh NG]]
[[Category: Malatesta, F.]]
[[Category: Miele AE]]
[[Category: Marsh, N.G.]]
[[Category: Savino C]]
[[Category: Miele, A.E.]]
[[Category: Schimperna G]]
[[Category: Savino, C.]]
[[Category: Sciara G]]
[[Category: Schimperna, G.]]
[[Category: Vallone B]]
[[Category: Sciara, G.]]
[[Category: Vallone, B.]]
[[Category: 1PG]]
[[Category: ADL]]
[[Category: acetyl dithranol]]
[[Category: actinorhodin]]
[[Category: aromatic polyketides]]
[[Category: dihydrokalafungin]]
[[Category: monooxygenase]]
[[Category: streptomyces coelicolor]]
 
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Retrieved from "https://proteopedia.org/1n5s"