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[[Image:2cnt.gif|left|200px]]<br />
<applet load="2cnt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2cnt, resolution 2.40&Aring;" />
'''RIMI- RIBOSOMAL S18 N-ALPHA-PROTEIN ACETYLTRANSFERASE IN COMPLEX WITH COENZYMEA.'''<br />


==About this Structure==
==RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with CoenzymeA.==
2CNT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with SO4, COA and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.128 2.3.1.128]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CNT OCA]].
<StructureSection load='2cnt' size='340' side='right'caption='[[2cnt]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
[[Category: Salmonella typhimurium]]
== Structural highlights ==
[[Category: Single protein]]
<table><tr><td colspan='2'>[[2cnt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNT FirstGlance]. <br>
[[Category: Bareich, D.C.]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
[[Category: Blanchard, J.S.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
[[Category: Vetting, M.W.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnt OCA], [https://pdbe.org/2cnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cnt RCSB], [https://www.ebi.ac.uk/pdbsum/2cnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cnt ProSAT]</span></td></tr>
[[Category: Yu, M.]]
</table>
[[Category: COA]]
== Function ==
[[Category: GOL]]
[https://www.uniprot.org/uniprot/RIMI_SALTY RIMI_SALTY] Acetylates the N-terminal alanine of ribosomal protein S18.[HAMAP-Rule:MF_02210]<ref>PMID:18596200</ref>
[[Category: SO4]]
== Evolutionary Conservation ==
[[Category: acetyltransferase]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: acyltransferase]]
Check<jmol>
[[Category: gcn5-n-acetyltransferase]]
  <jmolCheckbox>
[[Category: gnat]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnt_consurf.spt"</scriptWhenChecked>
[[Category: n-alpha acetylation]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: ribosomal protein]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: transferase]]
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cnt ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three ribosomal proteins L7, S5, and S18 are included in the rare subset of prokaryotic proteins that are known to be N(alpha)-acetylated. The GCN5-related N-acetyltransferase (GNAT) protein RimI, responsible for the N(alpha)-acetylation of the ribosomal protein S18, was cloned from Salmonella typhimurium LT2 (RimI(ST)), overexpressed, and purified to homogeneity. Steady-state kinetic parameters for RimI(ST) were determined for AcCoA and a peptide substrate consisting of the first six amino acids of the target protein S18. The crystal structure of RimI(ST) was determined in complex with CoA, AcCoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor. The structures are consistent with a direct nucleophilic addition-elimination mechanism with Glu103 and Tyr115 acting as the catalytic base and acid, respectively. The RimI(ST)-bisubstrate complex suggests that several residues change conformation upon interacting with the N terminus of S18, including Glu103, the proposed active site base, facilitating proton exchange and catalysis.


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:25:07 2007''
Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18.,Vetting MW, Bareich DC, Yu M, Blanchard JS Protein Sci. 2008 Oct;17(10):1781-90. Epub 2008 Jul 2. PMID:18596200<ref>PMID:18596200</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2cnt" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ribosomal protein S18|Ribosomal protein S18]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
[[Category: Bareich DC]]
[[Category: Blanchard JS]]
[[Category: Vetting MW]]
[[Category: Yu M]]
Retrieved from "https://proteopedia.org/2cnt"