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New page: left|200px<br /><applet load="1na3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1na3, resolution 1.55Å" /> '''Design of Stable alp...
 
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[[Image:1na3.jpg|left|200px]]<br /><applet load="1na3" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1na3, resolution 1.55&Aring;" />
'''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br />


==Overview==
==Design of Stable alpha-Helical Arrays from an Idealized TPR Motif==
The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif, that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to, mediate protein-protein interactions. The binding specificity of each TPR, protein is different, although the underlying structural motif is the, same. Here we describe a statistical approach to the design of an, idealized TPR motif. We present the high-resolution X-ray crystal, structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their, solution properties and stability. A detailed analysis of these structures, provides an understanding of the TPR motif, how it is repeated to give, helical arrays with different superhelical twists, and how a very stable, framework may be constructed for future functional designs.
<StructureSection load='1na3' size='340' side='right'caption='[[1na3]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1na3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NA3 FirstGlance]. <br>
1NA3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, TRS and IPT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NA3 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPT:ISOPROPYL-1-BETA-D-THIOGALACTOSIDE'>IPT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1na3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1na3 OCA], [https://pdbe.org/1na3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1na3 RCSB], [https://www.ebi.ac.uk/pdbsum/1na3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1na3 ProSAT]</span></td></tr>
Design of stable alpha-helical arrays from an idealized TPR motif., Main ER, Xiong Y, Cocco MJ, D'Andrea L, Regan L, Structure. 2003 May;11(5):497-508. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737816 12737816]
</table>
[[Category: ]]
== Evolutionary Conservation ==
[[Category: Protein complex]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Andrea, L.D.]]
Check<jmol>
[[Category: Cocco, M.]]
  <jmolCheckbox>
[[Category: Main, E.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1na3_consurf.spt"</scriptWhenChecked>
[[Category: Regan, L.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Xiong, Y.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: IPT]]
  </jmolCheckbox>
[[Category: MG]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1na3 ConSurf].
[[Category: TRS]]
<div style="clear:both"></div>
[[Category: design]]
__TOC__
[[Category: tpr]]
</StructureSection>
 
[[Category: Large Structures]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:49:15 2007''
[[Category: Unidentified]]
[[Category: Cocco M]]
[[Category: D'Andrea L]]
[[Category: Main E]]
[[Category: Regan L]]
[[Category: Xiong Y]]
Retrieved from "https://proteopedia.org/1na3"