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New page: left|200px<br /><applet load="1eu8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eu8, resolution 1.9Å" /> '''STRUCTURE OF TREHALOS...
 
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[[Image:1eu8.gif|left|200px]]<br /><applet load="1eu8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1eu8, resolution 1.9&Aring;" />
'''STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS'''<br />


==Overview==
==STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS==
We report the crystallization and structure determination at 1.85 A of the, extracellular, membrane-anchored trehalose/maltose-binding protein (TMBP), in complex with its substrate trehalose. TMBP is the substrate recognition, site of the high-affinity trehalose/maltose ABC transporter of the, hyperthermophilic Archaeon Thermococcus litoralis. In vivo, this protein, is anchored to the membrane, presumably via an N-terminal cysteine lipid, modification. The crystallized protein was N-terminally truncated, resulting in a soluble protein exhibiting the same binding characteristics, as the wild-type protein. The protein shows the characteristic features of, a transport-related, substrate-binding protein and is structurally related, to the maltose-binding protein (MBP) of Escherichia coli. It consists of, two similar lobes, each formed by a parallel beta-sheet flanked by, alpha-helices on both sides. Both are connected by a hinge region, consisting of two antiparallel beta-strands and an alpha-helix. As in MBP, the substrate is bound in the cleft between the lobes by hydrogen bonds, and hydrophobic interactions. However, compared to maltose binding in MBP, direct hydrogen bonding between the substrate and the protein prevails, while apolar contacts are reduced. To elucidate factors contributing to, thermostability, we compared TMBP with its mesophilic counterpart MBP and, found differences known from similar investigations. Specifically, we find, helices that are longer than their structurally equivalent counterparts, and fewer internal cavities.
<StructureSection load='1eu8' size='340' side='right'caption='[[1eu8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
1EU8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis] with PT, CL and TRE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900006:trehalose'>PRD_900006</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [https://pdbe.org/1eu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [https://www.ebi.ac.uk/pdbsum/1eu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eu8 ProSAT]</span></td></tr>
The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85 A., Diez J, Diederichs K, Greller G, Horlacher R, Boos W, Welte W, J Mol Biol. 2001 Jan 26;305(4):905-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11162101 11162101]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/MALE_THELN MALE_THELN] Part of the ABC transporter complex MalEFGK involved in trehalose/maltose import. Binds maltose and trehalose.<ref>PMID:11453995</ref> <ref>PMID:16532450</ref> <ref>PMID:8759837</ref> <ref>PMID:9457875</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1eu8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eu8 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermococcus litoralis]]
[[Category: Thermococcus litoralis]]
[[Category: Boos, W.]]
[[Category: Boos W]]
[[Category: Diederichs, K.]]
[[Category: Diederichs K]]
[[Category: Diez, J.]]
[[Category: Diez J]]
[[Category: Greller, G.]]
[[Category: Greller G]]
[[Category: Horlacher, R.]]
[[Category: Horlacher R]]
[[Category: Welte, W.]]
[[Category: Welte W]]
[[Category: CL]]
[[Category: PT]]
[[Category: TRE]]
[[Category: abc transporter fold]]
[[Category: mbp 2 fold]]
[[Category: protein-carbohydrate complex]]
[[Category: thermophilic protein]]
[[Category: trehalose-maltose binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:49:36 2007''

Latest revision as of 07:07, 7 February 2024

STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS

1eu8, resolution 1.90Å

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