1izc: Difference between revisions
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New page: left|200px<br /><applet load="1izc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1izc, resolution 1.7Å" /> '''Crystal Structure Ana... |
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== | ==Crystal Structure Analysis of Macrophomate synthase== | ||
The Diels-Alder reaction, which forms a six-membered ring from an alkene | <StructureSection load='1izc' size='340' side='right'caption='[[1izc]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1izc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrophoma_commelinae Macrophoma commelinae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IZC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1izc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1izc OCA], [https://pdbe.org/1izc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1izc RCSB], [https://www.ebi.ac.uk/pdbsum/1izc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1izc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9UVD4_9PEZI Q9UVD4_9PEZI] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/1izc_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1izc ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Diels-Alder reaction, which forms a six-membered ring from an alkene (dienophile) and a 1,3-diene, is synthetically very useful for construction of cyclic products with high regio- and stereoselectivity under mild conditions. It has been applied to the synthesis of complex pharmaceutical and biologically active compounds. Although evidence on natural Diels-Alderases has been accumulated in the biosynthesis of secondary metabolites, there has been no report on the structural details of the natural Diels-Alderases. The function and catalytic mechanism of the natural Diels-Alderase are of great interest owing to the diversity of molecular skeletons in natural Diels-Alder adducts. Here we present the 1.70 A resolution crystal structure of the natural Diels-Alderase, fungal macrophomate synthase (MPS), in complex with pyruvate. The active site of the enzyme is large and hydrophobic, contributing amino acid residues that can hydrogen-bond to the substrate 2-pyrone. These data provide information on the catalytic mechanism of MPS, and suggest that the reaction proceeds via a large-scale structural reorganization of the product. | |||
Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase.,Ose T, Watanabe K, Mie T, Honma M, Watanabe H, Yao M, Oikawa H, Tanaka I Nature. 2003 Mar 13;422(6928):185-9. PMID:12634789<ref>PMID:12634789</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1izc" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Macrophoma commelinae]] | [[Category: Macrophoma commelinae]] | ||
[[Category: Honma M]] | |||
[[Category: Honma | [[Category: Mie T]] | ||
[[Category: Mie | [[Category: Oikawa H]] | ||
[[Category: Oikawa | [[Category: Ose T]] | ||
[[Category: Ose | [[Category: Tanaka I]] | ||
[[Category: Tanaka | [[Category: Watanabe H]] | ||
[[Category: Watanabe | [[Category: Watanabe K]] | ||
[[Category: Watanabe | [[Category: Yao M]] | ||
[[Category: Yao | |||
Latest revision as of 05:54, 6 August 2025
Crystal Structure Analysis of Macrophomate synthase
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