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New page: left|200px<br /><applet load="1agl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1agl, resolution 2.200Å" /> '''STRUCTURE OF A DNA-...
 
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[[Image:1agl.gif|left|200px]]<br /><applet load="1agl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1agl, resolution 2.200&Aring;" />
'''STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX'''<br />


==Overview==
==STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX==
The application of detailed structural data bases has now culminated in, the successful design of a new generation of bisanthracyclines that form, ultratight DNA complexes [Chaires, J. B., Leng, F., Przewloka, T., Fokt, I., Ling, Y. H., Perez-Soler, R., &amp; Priebe, W. (1997) J. Med. Chem. 40, 261-266]. Daunomycin dimers were designed to bind to DNA in complexes, resembling those of monomers intercalated at adjacent sites. The goal of, the work described here was to determine, with X-ray crystallography, if a, potent member of this newly designed and synthesized class of, bisanthracyclines (WP631) binds as intended. WP631 is composed of two, daunomycin molecules, linked N3' to N3' by a xylyl group. We have solved, the 2.2 A X-ray crystal structure of a complex of WP631 bound to, [d(CGATCG)]2. We demonstrate, on a detailed molecular level, that the, WP631 design strategy is a success. The structures of WP631 and two, daunomycin molecules bound to [d(CGATCG)]2 provide the unprecedented, opportunity for detailed comparison of mono- and bis-intercalated, complexes of the same chromophore, allowing us to distinguish effects of, mono-intercalation from those of bis-intercalation. Differences are, focused primarily in the centers of the complexes. DNA unwinding and other, helical distortions propagate more efficiently to the center of the WP631, complex than to the center of the daunomycin complex.
<StructureSection load='1agl' size='340' side='right'caption='[[1agl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1agl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AGL FirstGlance]. <br>
1AGL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with BDA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AGL OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDA:4-METHYLBENZYL-N-BIS[DAUNOMYCIN]'>BDA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1agl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1agl OCA], [https://pdbe.org/1agl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1agl RCSB], [https://www.ebi.ac.uk/pdbsum/1agl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1agl ProSAT]</span></td></tr>
Structure of a DNA-bisdaunomycin complex., Hu GG, Shui X, Leng F, Priebe W, Chaires JB, Williams LD, Biochemistry. 1997 May 20;36(20):5940-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9166763 9166763]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Chaires, J.B.]]
</StructureSection>
[[Category: Hu, G.G.]]
[[Category: Large Structures]]
[[Category: Leng, F.]]
[[Category: Chaires JB]]
[[Category: Priebe, W.]]
[[Category: Hu GG]]
[[Category: Shui, X.]]
[[Category: Leng F]]
[[Category: Williams, L.D.]]
[[Category: Priebe W]]
[[Category: BDA]]
[[Category: Shui X]]
[[Category: complexed with drug]]
[[Category: Williams LD]]
[[Category: double helix]]
[[Category: right handed dna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:50:33 2007''

Latest revision as of 06:30, 7 February 2024

STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX

1agl, resolution 2.20Å

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