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New page: left|200px<br /><applet load="1ne9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ne9, resolution 1.70Å" /> '''Crystal Structure of...
 
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[[Image:1ne9.jpg|left|200px]]<br /><applet load="1ne9" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ne9, resolution 1.70&Aring;" />
'''Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution'''<br />


==Overview==
==Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution==
Members of the FemABX protein family are novel therapeutic targets, as, they are involved in the synthesis of the bacterial cell wall. They, catalyze the addition of amino acid(s) on the peptidoglycan precursor, using aminoacylated tRNA as a substrate. We report here the, high-resolution structure of Weissella viridescens L-alanine transferase, FemX and its complex with the UDP-MurNAc-pentapeptide. This is the first, structure example of a FemABX family member that does not possess a, coiled-coil domain. FemX consists of two structurally equivalent domains, separated by a cleft containing the binding site of the, UDP-MurNAc-pentapeptide and a long channel that traverses one of the two, domains. Our structural studies bring new insights into the evolution of, the FemABX and the related GNAT superfamilies, shed light on the, recognition site of the aminoacylated tRNA in Fem proteins, and allowed, manual docking of the acceptor end of the alanyl-tRNAAla.
<StructureSection load='1ne9' size='340' side='right'caption='[[1ne9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ne9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NE9 FirstGlance]. <br>
1NE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NE9 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ne9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ne9 OCA], [https://pdbe.org/1ne9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ne9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ne9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ne9 ProSAT]</span></td></tr>
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition., Biarrotte-Sorin S, Maillard AP, Delettre J, Sougakoff W, Arthur M, Mayer C, Structure. 2004 Feb;12(2):257-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14962386 14962386]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/FEMX_WEIVI FEMX_WEIVI] Involved in the synthesis of the bacterial cell wall. Catalyzes the addition of alanine into the interchain peptide bridge of peptidoglycan precursor using aminoacyl-tRNA(Ala) as amino acid donor. This alanine is added to the epsilon-amino group of the L-lysine of the peptidoglycan UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, in a ribosome-independent mechanism (PubMed:11083873, PubMed:4248527, PubMed:12679335, PubMed:15901708, PubMed:23744707). Specific for UDP-N-acetyl-muramoyl-pentapeptide. Has no activity toward UDP-N-acetyl-muramoyl-tetrapeptide or UDP-N-acetyl-muramoyl-tripeptide (PubMed:15901708). Also acts on L-seryl-tRNA(Ser) (PubMed:4248527).<ref>PMID:11083873</ref> <ref>PMID:12679335</ref> <ref>PMID:15901708</ref> <ref>PMID:23744707</ref> <ref>PMID:4248527</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ne/1ne9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ne9 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Weissella viridescens]]
[[Category: Weissella viridescens]]
[[Category: Arthur, M.]]
[[Category: Arthur M]]
[[Category: Biarrotte-Sorin, S.]]
[[Category: Biarrotte-Sorin S]]
[[Category: Delettre, J.]]
[[Category: Delettre J]]
[[Category: Maillard, A.P.]]
[[Category: Maillard AP]]
[[Category: Mayer, C.]]
[[Category: Mayer C]]
[[Category: Sougakoff, W.]]
[[Category: Sougakoff W]]
[[Category: MG]]
[[Category: protein]]
 
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