1wc7: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(24 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1wc7.gif|left|200px]]<br />
<applet load="1wc7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wc7, resolution 2.33&Aring;" />
'''FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE'''<br />


==Overview==
==FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE==
The purification, crystallization and structure determination at 2.3 A, resolution of the complex of the pyridoxal-5'-phosphate (PLP) dependent, catalytic antibody 15A9 with a phosphopyridoxyl-L-alanine (PPL-L-alanine), substrate analogue are described. The crystal belongs to space group, P2(1), with two molecules in the asymmetric unit related by, non-crystallographic symmetry. The unit-cell parameters are a = 63.5, b =, 81.7, c = 79.3 A and beta is fortuitously 90 degrees . Refinement of the, structure converged at unacceptably high R factors. Although the, traditional analysis of intensity distribution did not indicate twinning, pseudomerohedral twinning was revealed by a newer test based on local, intensity differences [Padilla &amp; Yeates (2003), Acta Cryst. D59, 1124-1130]. When ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15805602 (full description)]]
<StructureSection load='1wc7' size='340' side='right'caption='[[1wc7]], [[Resolution|resolution]] 2.33&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wc7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WC7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.33&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PP3:ALANYL-PYRIDOXAL-5-PHOSPHATE'>PP3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wc7 OCA], [https://pdbe.org/1wc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wc7 RCSB], [https://www.ebi.ac.uk/pdbsum/1wc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wc7 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wc/1wc7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wc7 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1WC7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]] with IOD and PP3 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WC7 OCA]].
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of a pseudomerohedrally twinned monoclinic crystal form of a pyridoxal phosphate-dependent catalytic antibody., Golinelli-Pimpaneau B, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):472-6. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805602 15805602]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Christen P]]
[[Category: Christen, P.]]
[[Category: Golinelli-Pimpaneau B]]
[[Category: Golinelli-Pimpaneau, B.]]
[[Category: IOD]]
[[Category: PP3]]
[[Category: catalytic antibody]]
[[Category: phosphopyridoxyl-l-alanine]]
[[Category: pyridoxal-phosphate]]
[[Category: transamination]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:26:27 2007''

Latest revision as of 06:36, 1 May 2024

FAB FRAGMENT OF PLP-DEPENDENT CATALYTIC ANTIBODY 15A9 IN COMPLEX WITH PHOSPHOPYRIDOXYL-L-ALANINE

1wc7, resolution 2.33Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1wc7&oldid=4135228"