3hjy: Difference between revisions

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{{Seed}}
[[Image:3hjy.jpg|left|200px]]


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==Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA==
The line below this paragraph, containing "STRUCTURE_3hjy", creates the "Structure Box" on the page.
<StructureSection load='3hjy' size='340' side='right'caption='[[3hjy]], [[Resolution|resolution]] 3.65&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3hjy]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HJY FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hjy OCA], [https://pdbe.org/3hjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hjy RCSB], [https://www.ebi.ac.uk/pdbsum/3hjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hjy ProSAT]</span></td></tr>
{{STRUCTURE_3hjy|  PDB=3hjy  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRUB_PYRFU TRUB_PYRFU] Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/3hjy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hjy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Box H/ACA small nucleolar and Cajal body ribonucleoprotein particles comprise the most complex pseudouridine synthases and are essential for ribosome and spliceosome maturation. The multistep and multicomponent-mediated enzyme mechanism remains only partially understood. Here we report a crystal structure at 2.35 A of a substrate-bound functional archaeal enzyme containing three of the four proteins, Cbf5, Nop10 and L7Ae, and a box H/ACA RNA that reveals detailed information about the protein-only active site. The substrate RNA, containing 5-fluorouridine at the modification position, is fully docked and catalytically rearranged by the enzyme in a manner similar to that seen in two stand-alone pseudouridine synthases. Structural analysis provides a mechanism for plasticity in the diversity of guide RNA sequences used and identifies a substrate-anchoring loop of Cbf5 that also interacts with Gar1 in unliganded structures. Activity analyses of mutated proteins and RNAs support the structural findings and further suggest a role of the Cbf5 loop in regulation of enzyme activity.


===Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA===
Structure of a functional ribonucleoprotein pseudouridine synthase bound to a substrate RNA.,Liang B, Zhou J, Kahen E, Terns RM, Terns MP, Li H Nat Struct Mol Biol. 2009 Jul;16(7):740-6. Epub 2009 May 28. PMID:19478803<ref>PMID:19478803</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3hjy" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_19478803}}, adds the Publication Abstract to the page
*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
(as it appears on PubMed at http://www.pubmed.gov), where 19478803 is the PubMed ID number.
*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
-->
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
{{ABSTRACT_PUBMED_19478803}}
== References ==
 
<references/>
==About this Structure==
__TOC__
3HJY is a 5 chains structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HJY OCA].
</StructureSection>
 
[[Category: Large Structures]]
==Reference==
<ref group="xtra">PMID:19478803</ref><references group="xtra"/>
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Kahen, E.]]
[[Category: Kahen E]]
[[Category: Li, H.]]
[[Category: Li H]]
[[Category: Liang, B.]]
[[Category: Liang B]]
[[Category: Terns, M P.]]
[[Category: Terns MP]]
[[Category: Terns, R M.]]
[[Category: Terns RM]]
[[Category: Zhou, J.]]
[[Category: Zhou J]]
[[Category: Box h/aca]]
[[Category: Isomerase]]
[[Category: Isomerase/rna complex]]
[[Category: Post-transcriptional modification]]
[[Category: Protein-rna complex]]
[[Category: Pseudouridine synthase]]
[[Category: Pseudouridylase]]
[[Category: Pseudouridylation]]
[[Category: Ribonucleoprotein]]
[[Category: Ribonucleoprotein particle]]
[[Category: Ribosome biogenesis]]
[[Category: Rna editing]]
[[Category: Rnp]]
[[Category: Rrna processing]]
[[Category: Trna processing]]
 
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Retrieved from "https://proteopedia.org/3hjy"