1nly: Difference between revisions

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New page: left|200px<br /><applet load="1nly" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nly, resolution 2.80Å" /> '''Crystal structure of...
 
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[[Image:1nly.jpg|left|200px]]<br /><applet load="1nly" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nly, resolution 2.80&Aring;" />
'''Crystal structure of the traffic ATPase of the Helicobacter pylori type IV secretion system in complex with ATPgammaS'''<br />


==Overview==
==Crystal structure of the traffic ATPase of the Helicobacter pylori type IV secretion system in complex with ATPgammaS==
The coupling of ATP binding/hydrolysis to macromolecular secretion systems, is crucial to the pathogenicity of Gram-negative bacteria. We reported, previously the structure of the ADP-bound form of the hexameric traffic, VirB11 ATPase of the Helicobacter pylori type IV secretion system (named, HP0525), and proposed that it functions as a gating molecule at the inner, membrane, cycling through closed and open forms regulated by ATP, binding/hydrolysis. Here, we combine crystal structures with analytical, ultracentrifugation experiments to show that VirB11 ATPases indeed, function as dynamic hexameric assemblies. In the absence of nucleotide, the N-terminal domains exhibit a collection of rigid-body conformations., Nucleotide binding 'locks' the hexamer into a symmetric and compact, structure. We propose that VirB11s use the mechanical leverage generated, by such nucleotide-dependent conformational changes to facilitate the, export of substrates or the assembly of the type IV secretion apparatus., Biochemical characterization of mutant forms of HP0525 coupled with, electron microscopy and in vivo assays support such hypothesis, and, establish the relevance of VirB11s ATPases as drug targets against, pathogenic bacteria.
<StructureSection load='1nly' size='340' side='right'caption='[[1nly]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1nly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NLY FirstGlance]. <br>
1NLY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695] with MG, SO4, ATG and 2PE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NLY OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nly OCA], [https://pdbe.org/1nly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nly RCSB], [https://www.ebi.ac.uk/pdbsum/1nly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nly ProSAT]</span></td></tr>
VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion., Savvides SN, Yeo HJ, Beck MR, Blaesing F, Lurz R, Lanka E, Buhrdorf R, Fischer W, Haas R, Waksman G, EMBO J. 2003 May 1;22(9):1969-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12727865 12727865]
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7BK04_HELPX Q7BK04_HELPX]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nl/1nly_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nly ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Helicobacter pylori 26695]]
[[Category: Helicobacter pylori 26695]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Beck, M.R.]]
[[Category: Beck MR]]
[[Category: Blaesing, F.]]
[[Category: Blaesing F]]
[[Category: Buhrdorf, R.]]
[[Category: Buhrdorf R]]
[[Category: Fischer, W.]]
[[Category: Fischer W]]
[[Category: Haas, R.]]
[[Category: Haas R]]
[[Category: Lanka, E.]]
[[Category: Lanka E]]
[[Category: Lurz, R.]]
[[Category: Lurz R]]
[[Category: Savvides, S.N.]]
[[Category: Savvides SN]]
[[Category: Waksman, G.]]
[[Category: Waksman G]]
[[Category: Yeo, H.J.]]
[[Category: Yeo HJ]]
[[Category: 2PE]]
[[Category: ATG]]
[[Category: MG]]
[[Category: SO4]]
[[Category: atpgammas]]
[[Category: bacterial type iv secretion]]
[[Category: helicobacter pylori]]
[[Category: hp0525]]
[[Category: virb11 atpase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:22:09 2007''
Retrieved from "https://proteopedia.org/1nly"