2jjg: Difference between revisions

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{{Seed}}
[[Image:2jjg.jpg|left|200px]]


<!--
==Crystal structure of the M. tuberculosis Lysine-epsilon aminotransferase (Rv3290c) complexed to an inhibitor==
The line below this paragraph, containing "STRUCTURE_2jjg", creates the "Structure Box" on the page.
<StructureSection load='2jjg' size='340' side='right'caption='[[2jjg]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2jjg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JJG FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=L18:(2S)-1-METHYL-2-[(2S,4R)-2-METHYL-4-PHENYLPENTYL]PIPERIDINE'>L18</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
{{STRUCTURE_2jjg|  PDB=2jjg  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jjg OCA], [https://pdbe.org/2jjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jjg RCSB], [https://www.ebi.ac.uk/pdbsum/2jjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jjg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LAT_MYCTU LAT_MYCTU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/2jjg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jjg ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lysine varepsilon-aminotransferase (LAT) converts lysine to alpha-aminoadipate-delta-semialdehyde in a PLP-mediated reaction. We mutated active-site T330, N328 and E243, and structurally rationalized their properties. T330A and T330S mutants cannot bind PLP and are inactive. N328A although inactive, binds to PLP. E243A retains activity, but binds alpha-ketoglutarate in a different conformation. We had earlier identified 2-aminomethyl piperidine derivative as a LAT inhibitor. The co-crystal structure reveals that it mimics binding of C5 substrates and exhibits two binding modes. E243, that shields R422 in the apo enzyme, exhibits conformational changes to permit the binding of the inhibitor in one of the binding modes. Structure-based analysis of bound water in the active site suggests optimization strategies for synthesis of improved inhibitors.


===CRYSTAL STRUCTURE OF THE M. TUBERCULOSIS LYSINE-EPSILON AMINOTRANSFERASE (RV3290C) COMPLEXED TO AN INHIBITOR===
Mutational analysis of Mycobacterium tuberculosis lysine varepsilon-aminotransferase and inhibitor co-crystal structures, reveals distinct binding modes.,Tripathi SM, Agarwal A, Ramachandran R Biochem Biophys Res Commun. 2015 Jul 17-24;463(1-2):154-60. doi:, 10.1016/j.bbrc.2015.05.055. Epub 2015 May 20. PMID:26003725<ref>PMID:26003725</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2jjg" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
2JJG is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJG OCA].
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
[[Category: L-lysine 6-transaminase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Ramachandran, R.]]
[[Category: Ramachandran R]]
[[Category: Tripathi, S M.]]
[[Category: Tripathi SM]]
[[Category: Aminotransferase]]
[[Category: Inhibitor complex]]
[[Category: Lysine amino transferase]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Plp]]
[[Category: Pyridoxal phosphate]]
[[Category: Rv3290c]]
[[Category: Transferase]]
 
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Retrieved from "https://proteopedia.org/2jjg"