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New page: left|200px<br /><applet load="1ji3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ji3, resolution 2.20Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1ji3.gif|left|200px]]<br /><applet load="1ji3" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ji3, resolution 2.20&Aring;" />
'''CRYSTAL STRUCTURE OF THE FIRST THERMOSTABLE BACTERIAL LIPASE FROM BACILLUS STEAROTHERMOPHILUS'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE FIRST THERMOSTABLE BACTERIAL LIPASE FROM BACILLUS STEAROTHERMOPHILUS==
We describe the first lipase structure from a thermophilic organism. It, shares less than 20% amino acid sequence identity with other lipases for, which there are crystal structures, and shows significant insertions, compared with the typical alpha/beta hydrolase canonical fold. The, structure contains a zinc-binding site which is unique among all lipases, with known structures, and which may play a role in enhancing thermal, stability. Zinc binding is mediated by two histidine and two aspartic acid, residues. These residues are present in comparable positions in the, sequences of certain lipases for which there is as yet no crystal, structural information, such as those from Staphylococcal species and, Arabidopsis thaliana. The structure of Bacillus stearothermophilus P1, lipase provides a template for other thermostable lipases, and offers, insight into mechanisms used to enhance thermal stability which may be of, commercial value in engineering lipases for industrial uses.
<StructureSection load='1ji3' size='340' side='right'caption='[[1ji3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ji3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JI3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ji3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji3 OCA], [https://pdbe.org/1ji3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ji3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ji3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9L6D3_GEOSE Q9L6D3_GEOSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1ji3_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ji3 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JI3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with CA and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JI3 OCA].
*[[Lipase 3D Structures|Lipase 3D Structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of a thermostable lipase from Bacillus stearothermophilus P1., Tyndall JD, Sinchaikul S, Fothergill-Gilmore LA, Taylor P, Walkinshaw MD, J Mol Biol. 2002 Nov 8;323(5):859-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12417199 12417199]
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Triacylglycerol lipase]]
[[Category: Fothergill-Gilmore LA]]
[[Category: Fothergill-Gilmore, L.A.]]
[[Category: Sinchaikul S]]
[[Category: Sinchaikul, S.]]
[[Category: Taylor P]]
[[Category: Taylor, P.]]
[[Category: Tyndall JDA]]
[[Category: Tyndall, J.D.A.]]
[[Category: Walkinshaw MD]]
[[Category: Walkinshaw, M.D.]]
[[Category: CA]]
[[Category: ZN]]
[[Category: lipase]]
[[Category: metal-binding]]
[[Category: thermophilic]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:40:07 2007''

Latest revision as of 07:39, 7 February 2024

CRYSTAL STRUCTURE OF THE FIRST THERMOSTABLE BACTERIAL LIPASE FROM BACILLUS STEAROTHERMOPHILUS

1ji3, resolution 2.20Å

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