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New page: left|200px<br /><applet load="1nta" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nta, resolution 2.90Å" /> '''2.9 A crystal struct...
 
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[[Image:1nta.gif|left|200px]]<br /><applet load="1nta" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1nta, resolution 2.90&Aring;" />
'''2.9 A crystal structure of Streptomycin RNA-aptamer'''<br />


==Overview==
==2.9 A crystal structure of Streptomycin RNA-aptamer==
We describe a 2.9 A X-ray structure of a complex between the aminocyclitol, antibiotic streptomycin and an in vitro selected RNA aptamer, solved using, the anomalous diffraction properties of Ba cations. The RNA aptamer, which, contains two asymmetric internal loops, adopts a distinct, cation-stabilized fold involving a series of S-shaped backbone turns, anchored by canonical and noncanonical pairs and triples. The streptomycin, streptose ring is encapsulated by stacked arrays of bases from both loops, at the elbow of the L-shaped RNA architecture. Specificity is defined by, direct hydrogen bonds between all streptose functional groups and base, edges that line the inner walls of the cylindrical binding pocket. By, contrast, the majority of intermolecular interactions involve contacts to, backbone phosphates in the published structure of streptomycin bound to, the 16S rRNA.
<StructureSection load='1nta' size='340' side='right'caption='[[1nta]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1nta]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTA FirstGlance]. <br>
1NTA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with BA, NA and SRY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NTA OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SRY:STREPTOMYCIN'>SRY</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nta OCA], [https://pdbe.org/1nta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nta RCSB], [https://www.ebi.ac.uk/pdbsum/1nta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nta ProSAT]</span></td></tr>
Encapsulating streptomycin within a small 40-mer RNA., Tereshko V, Skripkin E, Patel DJ, Chem Biol. 2003 Feb;10(2):175-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12618190 12618190]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Patel, D.J.]]
</StructureSection>
[[Category: Skripkin, E.]]
[[Category: Large Structures]]
[[Category: Tereshko, V.]]
[[Category: Patel DJ]]
[[Category: BA]]
[[Category: Skripkin E]]
[[Category: NA]]
[[Category: Tereshko V]]
[[Category: SRY]]
[[Category: barium form]]
[[Category: streptomycin rna-aptamer]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:46:14 2007''

Latest revision as of 07:59, 14 February 2024

2.9 A crystal structure of Streptomycin RNA-aptamer

1nta, resolution 2.90Å

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