3i8c: Difference between revisions
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New page: '''Unreleased structure''' The entry 3i8c is ON HOLD Authors: Li, Ti., Robert, Eva I., Breugel, Pieter C. V., Strubin, Michel., Zheng, Ning. Description: Crystal Structure of DDB1 in C... |
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The | ==Crystal Structure of DDB1 in Complex with the H-Box Motif of WDR21A== | ||
<StructureSection load='3i8c' size='340' side='right'caption='[[3i8c]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3i8c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I8C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i8c OCA], [https://pdbe.org/3i8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i8c RCSB], [https://www.ebi.ac.uk/pdbsum/3i8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i8c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/DCAF4_HUMAN DCAF4_HUMAN] May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.<ref>PMID:16949367</ref> <ref>PMID:16964240</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/3i8c_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i8c ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cullin 4-DNA-damage-binding protein 1 (CUL4-DDB1) ubiquitin ligase machinery regulates diverse cellular functions and can be subverted by pathogenic viruses. Here we report the crystal structure of DDB1 in complex with a central fragment of hepatitis B virus X protein (HBx), whose DDB1-binding activity is important for viral infection. The structure reveals that HBx binds DDB1 through an alpha-helical motif, which is also found in the unrelated paramyxovirus SV5-V protein despite their sequence divergence. Our structure-based functional analysis suggests that, like SV5-V, HBx captures DDB1 to redirect the ubiquitin ligase activity of the CUL4-DDB1 E3 ligase. We also identify the alpha-helical motif shared by these viral proteins in the cellular substrate-recruiting subunits of the E3 complex, the DDB1-CUL4-associated factors (DCAFs) that are functionally mimicked by the viral hijackers. Together, our studies reveal a common yet promiscuous structural element that is important for the assembly of cellular and virally hijacked CUL4-DDB1 E3 complexes. | |||
A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery.,Li T, Robert EI, van Breugel PC, Strubin M, Zheng N Nat Struct Mol Biol. 2010 Jan;17(1):105-11. Epub 2009 Dec 6. PMID:19966799<ref>PMID:19966799</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3i8c" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[DNA damage-binding protein|DNA damage-binding protein]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Breugel PCV]] | |||
[[Category: Li T]] | |||
[[Category: Robert EI]] | |||
[[Category: Strubin M]] | |||
[[Category: Zheng N]] | |||
Latest revision as of 06:19, 27 November 2024
Crystal Structure of DDB1 in Complex with the H-Box Motif of WDR21A
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