2kk5: Difference between revisions
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< | ==High Fidelity Base Pairing at the 3'-Terminus== | ||
<StructureSection load='2kk5' size='340' side='right'caption='[[2kk5]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kk5]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KK5 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AU:2-AMINOURIDINE'>2AU</scene>, <scene name='pdbligand=AQC:9,10-DIOXO-9,10-DIHYDROANTHRACENE-2-CARBOXAMIDE'>AQC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kk5 OCA], [https://pdbe.org/2kk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kk5 RCSB], [https://www.ebi.ac.uk/pdbsum/2kk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kk5 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Binding target strands with single base selectivity at a terminal position is difficult with natural DNA or RNA hybridization probes. Nature uses a degenerate genetic code that is based on RNA:RNA codon:anticodon duplexes tolerating wobble base pairs at the terminus. The importance of short RNA strands in regulatory processes in the cell make it desirable to develop receptor-like approaches for high fidelity binding, even at the very 3'-terminus of a probe. Here, we report the three-dimensional structure of a DNA duplex with a 3'-terminal 2'-anthraquinoylamido-2'-deoxyuridine (Uaq) residue that was solved by NMR and restrained molecular dynamics. The Uaq residue binds the 5'-terminus of the target strand through a combination of pi-stacking, hydrogen bonding, and interactions in the minor groove. The acylated aminonucleoside is the best molecular cap for 3'-termini reported to date. The Uaq motif assists binding of DNA strands, but is particularly effective in enhancing the affinity for RNA target strands, with a DeltaT(m) in the UV melting point of up to +18.2 degrees C per residue. Increased base pairing selectivity is induced for all sequence motifs tested, even in cases where unmodified duplexes show no preference for the canonical base pair at all. A single mismatched nucleobase facing the 3'-terminus gives DeltaDeltaT(m) values as large as -23.9 degrees C (RNA) or -29.5 degrees C (DNA). The 5'-phosphoramidite of the Uaq cap reported here allows for routine incorporation during automated syntheses. | |||
High fidelity base pairing at the 3'-terminus.,Patra A, Richert C J Am Chem Soc. 2009 Sep 9;131(35):12671-81. PMID:19722718<ref>PMID:19722718</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
== | </div> | ||
<div class="pdbe-citations 2kk5" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Patra A]] | |||
[[Category: Richert C]] | |||
Latest revision as of 12:51, 20 December 2023
High Fidelity Base Pairing at the 3'-Terminus
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