1s7n: Difference between revisions

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New page: left|200px<br /><applet load="1s7n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s7n, resolution 2.1Å" /> '''Ribosomal L7/L12 alph...
 
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[[Image:1s7n.gif|left|200px]]<br /><applet load="1s7n" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1s7n, resolution 2.1&Aring;" />
'''Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA free sulfhydryl)'''<br />


==Overview==
==Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA free sulfhydryl)==
RimL is responsible for converting the prokaryotic ribosomal protein from, L12 to L7 by acetylation of its N-terminal amino group. We demonstrate, that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the, apostructure of RimL from Salmonella typhimurium and its complex with, coenzyme A, revealing a homodimeric oligomer with structural similarity to, other Gcn5-related N-acetyltransferase superfamily members. A large, central trough located at the dimer interface provides sufficient room to, bind both L12 N-terminal helices. Structural and biochemical analysis, indicates that RimL proceeds by single-step transfer rather than a, covalent-enzyme intermediate. This is the first structure of a, Gcn5-related N-acetyltransferase family member with demonstrated activity, toward a protein N(alpha)-amino group and is a first step toward, understanding the molecular basis for N(alpha)acetylation and its function, in cellular regulation.
<StructureSection load='1s7n' size='340' side='right'caption='[[1s7n]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1s7n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7N FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7n OCA], [https://pdbe.org/1s7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7n RCSB], [https://www.ebi.ac.uk/pdbsum/1s7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7n ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8ZPC0_SALTY Q8ZPC0_SALTY]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s7/1s7n_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s7n ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.


==About this Structure==
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium.,Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:15817456<ref>PMID:15817456</ref>
1S7N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2] with COA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S7N OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15817456 15817456]
</div>
[[Category: Salmonella typhimurium lt2]]
<div class="pdbe-citations 1s7n" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Blanchard, J.S]]
<references/>
[[Category: Carvalho, L.P.de.]]
__TOC__
[[Category: Roderick, S.L.]]
</StructureSection>
[[Category: Vetting, M.W.]]
[[Category: Large Structures]]
[[Category: COA]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
[[Category: acetyltransferase]]
[[Category: Blanchard JS]]
[[Category: alpha-n-protein acetyltransferase]]
[[Category: Roderick SL]]
[[Category: coenzyme a]]
[[Category: Vetting MW]]
[[Category: gnat]]
[[Category: De Carvalho LP]]
[[Category: l7/l12]]
 
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Retrieved from "https://proteopedia.org/1s7n"