1fml: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1fml" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fml, resolution 2.75Å" /> '''CRYSTAL STRUCTURE OF...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1fml.gif|left|200px]]<br /><applet load="1fml" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fml, resolution 2.75&Aring;" />
'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP'''<br />


==Overview==
==CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP==
We report here the crystal structure of retinol dehydratase, an enzyme, that catalyzes the synthesis of anhydroretinol. The enzyme is a member of, the sulfotransferase superfamily and its crystal structure reveals the, insertion of a helical lid into a canonical sulfotransferase fold., Site-directed mutations demonstrate that this inserted lid is necessary, for anhydroretinol production but not for sulfonation; thus, insertion of, a helical lid can convert a sulfotransferase into a dehydratase.
<StructureSection load='1fml' size='340' side='right'caption='[[1fml]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1fml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FML FirstGlance]. <br>
1FML is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda] with CA, A3P and RTL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=RTL:RETINOL'>RTL</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fml OCA], [https://pdbe.org/1fml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fml RCSB], [https://www.ebi.ac.uk/pdbsum/1fml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fml ProSAT]</span></td></tr>
A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11323722 11323722]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/Q26490_SPOFR Q26490_SPOFR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/1fml_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fml ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Spodoptera frugiperda]]
[[Category: Spodoptera frugiperda]]
[[Category: Buck, J.]]
[[Category: Buck J]]
[[Category: Kobayashi, M.]]
[[Category: Kobayashi M]]
[[Category: Newcomer, M.E.]]
[[Category: Newcomer ME]]
[[Category: Pakhomova, S.]]
[[Category: Pakhomova S]]
[[Category: A3P]]
[[Category: CA]]
[[Category: RTL]]
[[Category: 5'-diphosphate]]
[[Category: adenosine 3']]
[[Category: dehydratase]]
[[Category: retinol]]
[[Category: sulfotransferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:03:28 2007''
Retrieved from "https://proteopedia.org/1fml"