1s8n: Difference between revisions
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New page: left|200px<br /><applet load="1s8n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s8n, resolution 1.482Å" /> '''Crystal structure o... |
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== | ==Crystal structure of Rv1626 from Mycobacterium tuberculosis== | ||
We describe the crystal structure of Rv1626 from Mycobacterium | <StructureSection load='1s8n' size='340' side='right'caption='[[1s8n]], [[Resolution|resolution]] 1.48Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1s8n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S8N FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.482Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s8n OCA], [https://pdbe.org/1s8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s8n RCSB], [https://www.ebi.ac.uk/pdbsum/1s8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s8n ProSAT], [https://www.topsan.org/Proteins/XMTB/1s8n TOPSAN]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PDTAR_MYCTU PDTAR_MYCTU] Member of the two-component regulatory system pdtaR/pdtaS.<ref>PMID:16026786</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s8/1s8n_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s8n ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii. | |||
The crystal and solution structure of a putative transcriptional antiterminator from Mycobacterium tuberculosis.,Morth JP, Feng V, Perry LJ, Svergun DI, Tucker PA Structure. 2004 Sep;12(9):1595-605. PMID:15341725<ref>PMID:15341725</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1s8n" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Feng | <references/> | ||
[[Category: Morth | __TOC__ | ||
[[Category: Perry | </StructureSection> | ||
[[Category: Svergun | [[Category: Large Structures]] | ||
[[Category: Mycobacterium tuberculosis H37Rv]] | |||
[[Category: Tucker | [[Category: Feng V]] | ||
[[Category: Morth JP]] | |||
[[Category: Perry LJ]] | |||
[[Category: Svergun DI]] | |||
[[Category: Tucker PA]] | |||
Latest revision as of 06:14, 23 August 2023
Crystal structure of Rv1626 from Mycobacterium tuberculosis
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