1s9b: Difference between revisions

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New page: left|200px<br /><applet load="1s9b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9b, resolution 2.81Å" /> '''Crystal Structure An...
 
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[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1s9b, resolution 2.81&Aring;" />
'''Crystal Structure Analysis of the B-DNA GAATTCG'''<br />


==Overview==
==Crystal Structure Analysis of the B-DNA GAATTCG==
DNA oligonucleotides can be used in order to assemble highly structured, materials. Oligonucleotides with sticky ends can form long linear, structures, whereas branching is required to form two- and, three-dimensional nanostructures. In this paper, we show that when Ni(2+), is attached to the N7 atom of guanine, it can also act as a branching, point. Thus, we have found that the heptanucleotide d(GAATTCG) can, assemble into long linear duplex structures, which cross in space to, generate a cubic structure. The three-dimensional arrays are stabilized by, phosphate-Ni(2+)-guanine interactions. For the first time, the, crystallization of a B form DNA oligonucleotide in a cubic system is, reported, space group I23. Large solvent cavities are found among the DNA, duplexes.
<StructureSection load='1s9b' size='340' side='right'caption='[[1s9b]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1s9b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S9B FirstGlance]. <br>
1S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9b OCA], [https://pdbe.org/1s9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s9b RCSB], [https://www.ebi.ac.uk/pdbsum/1s9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s9b ProSAT]</span></td></tr>
A cubic arrangement of DNA double helices based on nickel-guanine interactions., Valls N, Uson I, Gouyette C, Subirana JA, J Am Chem Soc. 2004 Jun 30;126(25):7812-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15212528 15212528]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Gouyette, C.]]
</StructureSection>
[[Category: Subirana, J.A.]]
[[Category: Large Structures]]
[[Category: Uson, I.]]
[[Category: Gouyette C]]
[[Category: Valls, N.]]
[[Category: Subirana JA]]
[[Category: NI]]
[[Category: Uson I]]
[[Category: cubic dna]]
[[Category: Valls N]]
[[Category: double helix]]
[[Category: nickel]]
 
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