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New page: left|200px<br /><applet load="1wmw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wmw, resolution 1.55Å" /> '''Crystal structure of...
 
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[[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wmw, resolution 1.55&Aring;" />
'''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''<br />


==Overview==
==Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus==
Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8, was expressed in Escherichia coli, purified to homogeneity and, crystallized both as the recombinant native protein and its, selenomethionine (SeMet) derivative. Well diffracting crystals of these, proteins were obtained belonging to the tetragonal space group P4(1) or, P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were, two homodimers in the asymmetric unit. A native data set was collected to, 1.55 A resolution and a data set suitable for MAD phasing was collected to, 2.40 A resolution on beamline BL40B2 at SPring-8.
<StructureSection load='1wmw' size='340' side='right'caption='[[1wmw]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1wmw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WMW FirstGlance]. <br>
1WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wmw OCA], [https://pdbe.org/1wmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wmw RCSB], [https://www.ebi.ac.uk/pdbsum/1wmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wmw ProSAT], [https://www.topsan.org/Proteins/RSGI/1wmw TOPSAN]</span></td></tr>
==Reference==
</table>
Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8., Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684922 14684922]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q5SMD0_THET8 Q5SMD0_THET8]  
[[Category: Thermus thermophilus]]
== Evolutionary Conservation ==
[[Category: Hamada, K.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kawamoto, M.]]
Check<jmol>
[[Category: Kuramitu, S.]]
  <jmolCheckbox>
[[Category: Miura, K.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wm/1wmw_consurf.spt"</scriptWhenChecked>
[[Category: Nakagawa, N.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Nishio, K.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Nodake, Y.]]
  </jmolCheckbox>
[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wmw ConSurf].
[[Category: Suto, K.]]
<div style="clear:both"></div>
[[Category: ggpp]]
__TOC__
[[Category: prenyl diphosphate synthase]]
</StructureSection>
[[Category: riken structural genomics/proteomics initiative]]
[[Category: Large Structures]]
[[Category: rsgi]]
[[Category: Thermus thermophilus HB8]]
[[Category: structural genomics]]
[[Category: Hamada K]]
[[Category: thermus]]
[[Category: Kawamoto M]]
 
[[Category: Kuramitu S]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:17:00 2007''
[[Category: Miura K]]
[[Category: Nakagawa N]]
[[Category: Nishio K]]
[[Category: Nodake Y]]
[[Category: Suto K]]

Latest revision as of 13:32, 13 March 2024

Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus

1wmw, resolution 1.55Å

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