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New page: left|200px<br /><applet load="1sfu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sfu, resolution 2.0Å" /> '''Crystal structure of ...
 
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[[Image:1sfu.gif|left|200px]]<br /><applet load="1sfu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sfu, resolution 2.0&Aring;" />
'''Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA'''<br />


==Overview==
==Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA==
A conserved feature of poxviruses is a protein, well characterized as E3L, in vaccinia virus, that confers IFN resistance on the virus. This protein, comprises two domains, an N-terminal Z-DNA-binding protein domain (Zalpha), and a C-terminal double-stranded RNA-binding domain. Both are required for, pathogenicity of vaccinia virus in mice infected by intracranial, injection. Here, we describe the crystal structure of the Zalpha domain, from the E3L-like protein of Yaba-like disease virus, a Yatapoxvirus, in a, complex with Z-DNA, solved at a 2.0-A resolution. The DNA contacting, surface of Yaba-like disease virus Zalpha(E3L) closely resembles that of, other structurally defined members of the Zalpha family, although some, variability exists in the beta-hairpin region. In contrast to the, Z-DNA-contacting surface, the nonbinding surface of members of the Zalpha, family are unrelated; this surface may effect protein-specific, interactions. The presence of the conserved and tailored Z-DNA-binding, surface, which interacts specifically with the zigzag backbone and syn, base diagnostic of the Z-form, reinforces the importance to poxvirus, infection of the ability of this protein to recognize the Z-conformation.
<StructureSection load='1sfu' size='340' side='right'caption='[[1sfu]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1sfu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yaba-like_disease_virus Yaba-like disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfu OCA], [https://pdbe.org/1sfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfu RCSB], [https://www.ebi.ac.uk/pdbsum/1sfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/E3_YLDV E3_YLDV] RNA-binding protein that plays a role in the inhibition of multiple cellular antiviral responses activated by double-stranded RNA (dsRNA), such as inhibition of PKR activation, necroptosis, and IFN-mediated antiviral activities (By similarity). Recognizes and binds Z-RNA structures via its Z-binding domain and dsRNA via its DRBM domain: RNA-binding activity is required to escape host ZBP1-dependent necroptosis (By similarity). Mechanistically, the Z-binding domain binds Z-RNAs that are produced during Yaba-like disease virus infection, thereby competing with Z-RNA detection by host ZBP1, suppressing ZBP1-dependent necroptosis (By similarity).[UniProtKB:P21605]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sfu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfu ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1SFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SFU OCA].
*[[Z-DNA|Z-DNA]]
 
__TOC__
==Reference==
</StructureSection>
A poxvirus protein forms a complex with left-handed Z-DNA: crystal structure of a Yatapoxvirus Zalpha bound to DNA., Ha SC, Lokanath NK, Van Quyen D, Wu CA, Lowenhaupt K, Rich A, Kim YG, Kim KK, Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14367-72. Epub 2004 Sep 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15448208 15448208]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Yaba-like disease virus]]
[[Category: Viruses]]
[[Category: Ha SC]]
[[Category: Ha, S.C.]]
[[Category: Kim KK]]
[[Category: Kim, K.K.]]
[[Category: Kim YG]]
[[Category: Kim, Y.G.]]
[[Category: Lowenhaupt K]]
[[Category: Lowenhaupt, K.]]
[[Category: Rich A]]
[[Category: Quyen, D.Van.]]
[[Category: Van Quyen D]]
[[Category: Rich, A.]]
[[Category: Wu CA]]
[[Category: Wu, C.A.]]
[[Category: protein/z-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:25:03 2007''

Latest revision as of 08:30, 14 February 2024

Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA

1sfu, resolution 2.00Å

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