2zzx: Difference between revisions

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{{Seed}}
[[Image:2zzx.jpg|left|200px]]


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==Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate==
The line below this paragraph, containing "STRUCTURE_2zzx", creates the "Structure Box" on the page.
<StructureSection load='2zzx' size='340' side='right'caption='[[2zzx]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zzx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZZX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
{{STRUCTURE_2zzx|  PDB=2zzx  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zzx OCA], [https://pdbe.org/2zzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zzx RCSB], [https://www.ebi.ac.uk/pdbsum/2zzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zzx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TLBP_THET8 TLBP_THET8] Part of the tripartite ATP-independent periplasmic (TRAP) transport system involved in the uptake of lactate. This protein specifically binds L-lactate.[UniProtKB:P37676]<ref>PMID:19631222</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/2zzx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zzx ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lactate is utilized in many biological processes, and its transport across biological membranes is mediated with various types of transporters. Here, we report the crystal structures of a lactate-binding protein of a TRAP (tripartite ATP-independent periplasmic) secondary transporter from Thermus thermophilus HB8. The folding of the protein is typical for a type II periplasmic solute-binding protein and forms a dimer in a back-to-back manner. One molecule of l-lactate is clearly identified in a cleft of the protein as a complex with a calcium ion. Detailed crystallographic and biochemical analyses revealed that the calcium ion can be removed from the protein and replaced with other divalent cations. This characterization of the structure of a protein binding with calcium lactate makes a significant contribution to our understanding of the mechanisms by which calcium and lactate are accommodated in cells.


===Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate===
Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate.,Akiyama N, Takeda K, Miki K J Mol Biol. 2009 Sep 25;392(3):559-65. Epub 2009 Jul 22. PMID:19631222<ref>PMID:19631222</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zzx" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
2ZZX is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZX OCA].
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:19631222</ref><references group="xtra"/>
__TOC__
[[Category: Thermus thermophilus]]
</StructureSection>
[[Category: Akiyama, N.]]
[[Category: Large Structures]]
[[Category: Miki, K.]]
[[Category: Thermus thermophilus HB8]]
[[Category: Takeda, K.]]
[[Category: Akiyama N]]
[[Category: Lactate]]
[[Category: Miki K]]
[[Category: Periplasmic substrate binding protein]]
[[Category: Takeda K]]
[[Category: Transport protein]]
[[Category: Trap transporter]]
 
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Retrieved from "https://proteopedia.org/2zzx"