2zzv: Difference between revisions

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{{Seed}}
[[Image:2zzv.jpg|left|200px]]


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==Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Calcium and Lactate==
The line below this paragraph, containing "STRUCTURE_2zzv", creates the "Structure Box" on the page.
<StructureSection load='2zzv' size='340' side='right'caption='[[2zzv]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zzv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZZV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>
{{STRUCTURE_2zzv|  PDB=2zzv  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zzv OCA], [https://pdbe.org/2zzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zzv RCSB], [https://www.ebi.ac.uk/pdbsum/2zzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zzv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TLBP_THET8 TLBP_THET8] Part of the tripartite ATP-independent periplasmic (TRAP) transport system involved in the uptake of lactate. This protein specifically binds L-lactate.[UniProtKB:P37676]<ref>PMID:19631222</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/2zzv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zzv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lactate is utilized in many biological processes, and its transport across biological membranes is mediated with various types of transporters. Here, we report the crystal structures of a lactate-binding protein of a TRAP (tripartite ATP-independent periplasmic) secondary transporter from Thermus thermophilus HB8. The folding of the protein is typical for a type II periplasmic solute-binding protein and forms a dimer in a back-to-back manner. One molecule of l-lactate is clearly identified in a cleft of the protein as a complex with a calcium ion. Detailed crystallographic and biochemical analyses revealed that the calcium ion can be removed from the protein and replaced with other divalent cations. This characterization of the structure of a protein binding with calcium lactate makes a significant contribution to our understanding of the mechanisms by which calcium and lactate are accommodated in cells.


===Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Calcium and Lactate===
Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate.,Akiyama N, Takeda K, Miki K J Mol Biol. 2009 Sep 25;392(3):559-65. Epub 2009 Jul 22. PMID:19631222<ref>PMID:19631222</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zzv" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_19631222}}, adds the Publication Abstract to the page
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 19631222 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19631222}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2ZZV is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZV OCA].
[[Category: Thermus thermophilus HB8]]
 
[[Category: Akiyama N]]
==Reference==
[[Category: Miki K]]
<ref group="xtra">PMID:19631222</ref><references group="xtra"/>
[[Category: Takeda K]]
[[Category: Thermus thermophilus]]
[[Category: Akiyama, N.]]
[[Category: Miki, K.]]
[[Category: Takeda, K.]]
[[Category: Calcium]]
[[Category: Lactate]]
[[Category: Periplasmic substrate binding protein]]
[[Category: Transport protein]]
[[Category: Trap transporter]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 12 12:47:34 2009''

Latest revision as of 08:52, 15 November 2023

Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Calcium and Lactate

2zzv, resolution 1.40Å

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