3ign: Difference between revisions
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< | ==Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a== | ||
<StructureSection load='3ign' size='340' side='right'caption='[[3ign]], [[Resolution|resolution]] 1.83Å' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3ign]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinobacter_nauticus_VT8 Marinobacter nauticus VT8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83Å</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2E:9,9-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d 3,2-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)'>C2E</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ign FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ign OCA], [https://pdbe.org/3ign PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ign RCSB], [https://www.ebi.ac.uk/pdbsum/3ign PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ign ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A1U3W3_MARN8 A1U3W3_MARN8] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/3ign_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ign ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Recent studies of signal transduction in bacteria have revealed a unique second messenger, bis-(3'-5')-cyclic dimeric GMP (c-di-GMP), which regulates transitions between motile states and sessile states, such as biofilms. C-di-GMP is synthesized from two GTP molecules by diguanylate cyclases (DGC). The catalytic activity of DGCs depends on a conserved GG(D/E)EF domain, usually part of a larger multi-domain protein organization. The domains other than the GG(D/E)EF domain often control DGC activation. This paper presents the 1.83 A crystal structure of an isolated catalytically competent GG(D/E)EF domain from the A1U3W3_MARAV protein from Marinobacter aquaeolei. Co-crystallization with GTP resulted in enzymatic synthesis of c-di-GMP. Comparison with previously solved DGC structures shows a similar orientation of c-di-GMP bound to an allosteric regulatory site mediating feedback inhibition of the enzyme. Biosynthesis of c-di-GMP in the crystallization reaction establishes that the enzymatic activity of this DGC domain does not require interaction with regulatory domains. | |||
Crystal structure of a catalytically active GG(D/E)EF diguanylate cyclase domain from Marinobacter aquaeolei with bound c-di-GMP product.,Vorobiev SM, Neely H, Yu B, Seetharaman J, Xiao R, Acton TB, Montelione GT, Hunt JF J Struct Funct Genomics. 2012 Sep;13(3):177-83. doi: 10.1007/s10969-012-9136-4., Epub 2012 Jul 29. PMID:22843345<ref>PMID:22843345</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3ign" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
*[[Diguanylate cyclase|Diguanylate cyclase]] | |||
[[Category: | == References == | ||
[[Category: Marinobacter | <references/> | ||
[[Category: Acton | __TOC__ | ||
[[Category: Ciccosanti | </StructureSection> | ||
[[Category: Foote | [[Category: Large Structures]] | ||
[[Category: Hunt | [[Category: Marinobacter nauticus VT8]] | ||
[[Category: Montelione | [[Category: Acton TB]] | ||
[[Category: Ciccosanti C]] | |||
[[Category: Neely | [[Category: Foote EL]] | ||
[[Category: Sahdev | [[Category: Hunt JF]] | ||
[[Category: Seetharaman | [[Category: Montelione GT]] | ||
[[Category: Tong | [[Category: Neely H]] | ||
[[Category: Vorobiev | [[Category: Sahdev S]] | ||
[[Category: Wang | [[Category: Seetharaman J]] | ||
[[Category: Xiao | [[Category: Tong L]] | ||
[[Category: Vorobiev S]] | |||
[[Category: Wang H]] | |||
[[Category: Xiao R]] | |||
Latest revision as of 09:17, 30 October 2024
Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a
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