3igo: Difference between revisions
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< | ==Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920== | ||
<StructureSection load='3igo' size='340' side='right'caption='[[3igo]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3igo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum_Iowa_II Cryptosporidium parvum Iowa II]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> | |||
- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3igo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3igo OCA], [https://pdbe.org/3igo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3igo RCSB], [https://www.ebi.ac.uk/pdbsum/3igo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3igo ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A3FQ16_CRYPI A3FQ16_CRYPI] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/3igo_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3igo ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Calcium-dependent protein kinases (CDPKs) have pivotal roles in the calcium-signaling pathway in plants, ciliates and apicomplexan parasites and comprise a calmodulin-dependent kinase (CaMK)-like kinase domain regulated by a calcium-binding domain in the C terminus. To understand this intramolecular mechanism of activation, we solved the structures of the autoinhibited (apo) and activated (calcium-bound) conformations of CDPKs from the apicomplexan parasites Toxoplasma gondii and Cryptosporidium parvum. In the apo form, the C-terminal CDPK activation domain (CAD) resembles a calmodulin protein with an unexpected long helix in the N terminus that inhibits the kinase domain in the same manner as CaMKII. Calcium binding triggers the reorganization of the CAD into a highly intricate fold, leading to its relocation around the base of the kinase domain to a site remote from the substrate binding site. This large conformational change constitutes a distinct mechanism in calcium signal-transduction pathways. | |||
Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium.,Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R Nat Struct Mol Biol. 2010 May;17(5):596-601. Epub 2010 May 2. PMID:20436473<ref>PMID:20436473</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3igo" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
*[[Calcium-dependent protein kinase|Calcium-dependent protein kinase]] | |||
[[Category: | == References == | ||
[[Category: Allali-Hassanali | <references/> | ||
[[Category: Amani | __TOC__ | ||
[[Category: Arrowsmith | </StructureSection> | ||
[[Category: Artz | [[Category: Cryptosporidium parvum Iowa II]] | ||
[[Category: Bochkarev | [[Category: Large Structures]] | ||
[[Category: Bountra | [[Category: Allali-Hassanali A]] | ||
[[Category: Edwards | [[Category: Amani M]] | ||
[[Category: Finnerty | [[Category: Arrowsmith CH]] | ||
[[Category: Hui | [[Category: Artz JD]] | ||
[[Category: Lin | [[Category: Bochkarev A]] | ||
[[Category: MacKenzie | [[Category: Bountra C]] | ||
[[Category: Edwards AM]] | |||
[[Category: Tempel | [[Category: Finnerty P]] | ||
[[Category: Vedadi | [[Category: Hui R]] | ||
[[Category: Weigelt | [[Category: Lin YH]] | ||
[[Category: Wernimont | [[Category: MacKenzie F]] | ||
[[Category: Tempel W]] | |||
[[Category: Vedadi M]] | |||
[[Category: Weigelt J]] | |||
[[Category: Wernimont AK]] | |||
Latest revision as of 07:47, 6 September 2023
Crystal structure of Cryptosporidium parvum CDPK1, cgd3_920
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