3i5w: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:3i5w.png|left|200px]]


<!--
==Crystal structure of human alpha-defensin 5 (mutant R13H)==
The line below this paragraph, containing "STRUCTURE_3i5w", creates the "Structure Box" on the page.
<StructureSection load='3i5w' size='340' side='right'caption='[[3i5w]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3i5w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I5W FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
{{STRUCTURE_3i5w|  PDB=3i5w  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i5w OCA], [https://pdbe.org/3i5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i5w RCSB], [https://www.ebi.ac.uk/pdbsum/3i5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i5w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEF5_HUMAN DEF5_HUMAN] Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.<ref>PMID:12021776</ref> <ref>PMID:15616305</ref> <ref>PMID:17088326</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Defensins constitute a major family of natural antimicrobial peptides that protect the host against microbial invasion. Here, we report on the antibacterial properties and cellular interaction of Human Defensin 5 as a function of its positive charge and hydrophobicity. We find that selective replacement of arginine residues in HD-5 by alanine or charge-neutral lysine residues reduces antibacterial killing as well as host cell interaction. We identify arginines at positions 9 and 28 in the HD-5 sequence as particularly important for its function. Replacement of arginine at position 13 to Histidine, as observed in a Crohn's disease patient, reduced bacterial killing strain-selectively. Finally, we find that HD-5 interacts with host cells via receptor-mediated mechanisms.


===Crystal structure of human alpha-defensin 5 (mutant R13H)===
Selective arginines are important for the antibacterial activity and host cell interaction of human alpha-defensin 5.,de Leeuw E, Rajabi M, Zou G, Pazgier M, Lu W FEBS Lett. 2009 Aug 6;583(15):2507-12. Epub 2009 Jul 7. PMID:19589339<ref>PMID:19589339</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3i5w" style="background-color:#fffaf0;"></div>


<!--
==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_19589339}}, adds the Publication Abstract to the page
*[[Defensin 3D structures|Defensin 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 19589339 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19589339}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Homo sapiens]]
3I5W is a 2 chains structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I5W OCA].
[[Category: Large Structures]]
 
[[Category: Lu W]]
==Reference==
[[Category: Pazgier M]]
<ref group="xtra">PMID:19589339</ref><references group="xtra"/>
[[Category: Lu, W.]]
[[Category: Pazgier, M.]]
[[Category: Antibiotic]]
[[Category: Antimicrobial]]
[[Category: Antimicrobial peptide]]
[[Category: Antimicrobial protein]]
[[Category: Defensin]]
[[Category: Disulfide bond]]
[[Category: Fungicide]]
[[Category: Hd5]]
[[Category: Human alpha-defensin 5]]
[[Category: Secreted]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 19 14:01:29 2009''

Latest revision as of 11:27, 2 August 2023

Crystal structure of human alpha-defensin 5 (mutant R13H)

3i5w, resolution 1.63Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=3i5w&oldid=3816680"