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New page: left|200px<br /><applet load="1k43" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k43" /> '''10 Structure Ensemble of the 14-residue pept...
 
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[[Image:1k43.gif|left|200px]]<br /><applet load="1k43" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k43" />
'''10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)'''<br />


==Overview==
==10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)==
Here we present a combinatorial approach to evolve a stable beta-hairpin, fold in a linear peptide. Starting with a de novo-designed linear peptide, that shows a beta-hairpin structure population of around 30%, we selected, four positions to build up a combinatorial library of 20(4) sequences., Deconvolution of the library using circular dichroism reduced such a, sequence complexity to 36 defined sequences. Circular dichroism and NMR of, these peptides resulted in the identification of two linear 14-aa-long, peptides that in plain buffered solutions showed a percentage of, beta-hairpin structure higher than 70%. Our results show how combinatorial, approaches can be used to obtain highly structured peptide sequences that, could be used as templates in which functionality can be introduced.
<StructureSection load='1k43' size='340' side='right'caption='[[1k43]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k43]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K43 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k43 OCA], [https://pdbe.org/1k43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k43 RCSB], [https://www.ebi.ac.uk/pdbsum/1k43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k43 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.


==About this Structure==
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides.,Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:11782528<ref>PMID:11782528</ref>
1K43 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K43 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides., Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E, Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11782528 11782528]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1k43" style="background-color:#fffaf0;"></div>
[[Category: Lacroix, E.]]
== References ==
[[Category: Pastor, M.T.]]
<references/>
[[Category: Paz, M.Lopez.de.la.]]
__TOC__
[[Category: Perez-Paya, E.]]
</StructureSection>
[[Category: Serrano, L.]]
[[Category: Large Structures]]
[[Category: beta-hairpin]]
[[Category: Lacroix E]]
 
[[Category: Lopez de la Paz M]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:41:59 2007''
[[Category: Pastor MT]]
[[Category: Perez-Paya E]]
[[Category: Serrano L]]
Retrieved from "https://proteopedia.org/1k43"