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New page: left|200px<br /><applet load="1k66" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k66, resolution 1.75Å" /> '''Crystal Structure of...
 
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[[Image:1k66.jpg|left|200px]]<br /><applet load="1k66" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k66, resolution 1.75&Aring;" />
'''Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB'''<br />


==Overview==
==Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB==
The structures of two response regulators (RRs) from the cyanobacterium, Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A, resolution, respectively. RcpA was found in phosphorylated and RcpB in, nonphosphorylated form. Both RRs are members of phytochrome-associated, light-sensing two-component signal transduction pathways, based on, histidine kinase-mediated receptor autophosphorylation and phosphorelay to, a RR. Despite the overall folding similarity to CheY-type RRs, ((beta/alpha)(5)-motif), RcpA and RcpB form homodimers, irrespective of, their phosphorylation state, giving insight into a signal transduction, putatively different from that of other known RRs. Dimerization is, accomplished by a C-terminal extension of the RR polypeptide chain, and, the surface formed by H4, beta 5, and H5, which constitute a hydrophobic, contact area with distinct interactions between residues of either, subunit. Sequence alignments reveal that the identified dimerization motif, is archetypal for phytochrome-associated RRs, making them a novel subgroup, of CheY-type RRs. The protein structures of RcpA and RcpB are compared to, the recently presented protein structure of Rcp1 from Synechocystis.
<StructureSection load='1k66' size='340' side='right'caption='[[1k66]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k66]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tolypothrix_sp._PCC_7601 Tolypothrix sp. PCC 7601]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K66 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k66 OCA], [https://pdbe.org/1k66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k66 RCSB], [https://www.ebi.ac.uk/pdbsum/1k66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k66 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RTM8_9CYAN Q8RTM8_9CYAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k66_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k66 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1K66 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tolypothrix_sp._pcc_7601 Tolypothrix sp. pcc 7601] with BME as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K66 OCA].
*[[Response regulator 3D structure|Response regulator 3D structure]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators., Benda C, Scheufler C, Tandeau de Marsac N, Gartner W, Biophys J. 2004 Jul;87(1):476-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15240481 15240481]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Tolypothrix sp. PCC 7601]]
[[Category: Tolypothrix sp. pcc 7601]]
[[Category: Benda C]]
[[Category: Benda, C.]]
[[Category: Gaertner W]]
[[Category: Gaertner, W.]]
[[Category: Scheufler C]]
[[Category: Marsac, N.Tandeau.de.]]
[[Category: Tandeau de Marsac N]]
[[Category: Scheufler, C.]]
[[Category: BME]]
[[Category: (beta/alpha)5]]
[[Category: apo-protein]]
[[Category: chey homologue]]
[[Category: homodimer]]
[[Category: response regulator]]
 
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Retrieved from "https://proteopedia.org/1k66"