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[[Image:1dyq.gif|left|200px]]<br />
<applet load="1dyq" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dyq, resolution 1.50&Aring;" />
'''STAPHYLOCOCCAL ENTEROTOXIN A MUTANT VACCINE'''<br />


==Overview==
==STAPHYLOCOCCAL ENTEROTOXIN A MUTANT VACCINE==
Staphylococcal enterotoxins (SEs) are superantigenic protein toxins, responsible for a number of life-threatening diseases. The X-ray structure, of a staphylococcal enterotoxin A (SEA) triple-mutant (L48R, D70R, and, Y92A) vaccine reveals a cascade of structural rearrangements located in, three loop regions essential for binding the alpha subunit of major, histocompatibility complex class II (MHC-II) molecules. A comparison of, hypothetical model complexes between SEA and the SEA triple mutant with, MHC-II HLA-DR1 clearly shows disruption of key ionic and hydrophobic, interactions necessary for forming the complex. Extensive dislocation of, the disulfide loop in particular interferes with MHC-IIalpha binding. The, triple-mutant structure provides new insights into the loss of, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11847286 (full description)]]
<StructureSection load='1dyq' size='340' side='right'caption='[[1dyq]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dyq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DYQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dyq OCA], [https://pdbe.org/1dyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dyq RCSB], [https://www.ebi.ac.uk/pdbsum/1dyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dyq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ETXA_STAAU ETXA_STAAU] Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome. The illness is characterized by high fever, hypotension, diarrhea, shock, and in some cases death.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dy/1dyq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dyq ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcal enterotoxins (SEs) are superantigenic protein toxins responsible for a number of life-threatening diseases. The X-ray structure of a staphylococcal enterotoxin A (SEA) triple-mutant (L48R, D70R, and Y92A) vaccine reveals a cascade of structural rearrangements located in three loop regions essential for binding the alpha subunit of major histocompatibility complex class II (MHC-II) molecules. A comparison of hypothetical model complexes between SEA and the SEA triple mutant with MHC-II HLA-DR1 clearly shows disruption of key ionic and hydrophobic interactions necessary for forming the complex. Extensive dislocation of the disulfide loop in particular interferes with MHC-IIalpha binding. The triple-mutant structure provides new insights into the loss of superantigenicity and toxicity of an engineered superantigen and provides a basis for further design of enterotoxin vaccines.


==About this Structure==
Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-IIalpha.,Krupka HI, Segelke BW, Ulrich RG, Ringhofer S, Knapp M, Rupp B Protein Sci. 2002 Mar;11(3):642-51. PMID:11847286<ref>PMID:11847286</ref>
1DYQ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]] with ZN and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: ZN1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DYQ OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-IIalpha., Krupka HI, Segelke BW, Ulrich RG, Ringhofer S, Knapp M, Rupp B, Protein Sci. 2002 Mar;11(3):642-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11847286 11847286]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1dyq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Krupka, H.I.]]
[[Category: Krupka HI]]
[[Category: Rupp, B.]]
[[Category: Rupp B]]
[[Category: Segelke, B.W.]]
[[Category: Segelke BW]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: enterotoxin]]
[[Category: staphylococcal enterotoxin type a]]
[[Category: vaccine]]
 
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Retrieved from "https://proteopedia.org/1dyq"