1c4l: Difference between revisions
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New page: left|200px<br /><applet load="1c4l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c4l" /> '''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDIN... |
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== | ==SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR== | ||
The formation of the C-U base pair in a duplex was observed in solution by | <StructureSection load='1c4l' size='340' side='right'caption='[[1c4l]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1c4l]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C4L FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c4l OCA], [https://pdbe.org/1c4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c4l RCSB], [https://www.ebi.ac.uk/pdbsum/1c4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c4l ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The formation of the C-U base pair in a duplex was observed in solution by means of the temperature profile of (15)N chemical shifts, and the precise geometry of the C-U base pair was also determined by NOE-based structure calculation. From the solution structure of the RNA oligomer, r[CGACUCAGG].r[CCUGCGUCG], it was found that a single C-U mismatch preferred being stacked in the duplex rather than being flipped-out even in solution. Moreover, it adopts an irregular geometry, where the amino nitrogen (N4) of the cytidine and keto-oxygen (O4) of the uridine are within hydrogen-bonding distance, as seen in crystals. To further prove the presence of a hydrogen bond in the C-U pair, we employed a point-labeled cytidine at the exocyclic amino nitrogen of the cytidine in the C-U pair. The temperature profile of its (15)N chemical shift showed a sigmoidal transition curve, indicating the presence of a hydrogen bond in the C-U pair in the duplex. | |||
Solution structure of an RNA duplex including a C-U base pair.,Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:10852704<ref>PMID:10852704</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1c4l" style="background-color:#fffaf0;"></div> | ||
[[Category: Kainosho | == References == | ||
[[Category: Kodama | <references/> | ||
[[Category: Kojima | __TOC__ | ||
[[Category: Kyogoku | </StructureSection> | ||
[[Category: Miyashita | [[Category: Large Structures]] | ||
[[Category: Ono | [[Category: Kainosho M]] | ||
[[Category: Ono | [[Category: Kodama TS]] | ||
[[Category: Tanaka | [[Category: Kojima C]] | ||
[[Category: Yamazaki | [[Category: Kyogoku Y]] | ||
[[Category: Yasuno | [[Category: Miyashita S]] | ||
[[Category: Ono AM]] | |||
[[Category: Ono AS]] | |||
[[Category: Tanaka Y]] | |||
[[Category: Yamazaki T]] | |||
[[Category: Yasuno K]] | |||
Latest revision as of 11:38, 22 November 2023
SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR
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