3hp4: Difference between revisions

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{{Seed}}
[[Image:3hp4.png|left|200px]]


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==Crystal structure of psychrotrophic esterase EstA from Pseudoalteromonas sp. 643A inhibited by monoethylphosphonate==
The line below this paragraph, containing "STRUCTURE_3hp4", creates the "Structure Box" on the page.
<StructureSection load='3hp4' size='340' side='right'caption='[[3hp4]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3hp4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_sp._643A Pseudoalteromonas sp. 643A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HP4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HP4 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MIR:MONOETHYLPHOSPHORYLSERINE'>MIR</scene></td></tr>
{{STRUCTURE_3hp4|  PDB=3hp4  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hp4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hp4 OCA], [https://pdbe.org/3hp4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hp4 RCSB], [https://www.ebi.ac.uk/pdbsum/3hp4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hp4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q1G1I7_9GAMM Q1G1I7_9GAMM]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/3hp4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hp4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the esterase EstA from the cold-adapted bacterium Pseudoalteromonas sp. 643A was determined in a covalently inhibited form at a resolution of 1.35 A. The enzyme has a typical SGNH hydrolase structure consisting of a single domain containing a five-stranded beta-sheet, with three helices at the convex side and two helices at the concave side of the sheet, and is ornamented with a couple of very short helices at the domain edges. The active site is located in a groove and contains the classic catalytic triad of Ser, His and Asp. In the structure of the crystal soaked in diethyl p-nitrophenyl phosphate (DNP), the catalytic serine is covalently connected to a phosphonate moiety that clearly has only one ethyl group. This is the only example in the Protein Data Bank of a DNP-inhibited enzyme with covalently bound monoethylphosphate.


===Crystal structure of psychrotrophic esterase EstA from Pseudoalteromonas sp. 643A inhibited by monoethylphosphonate===
Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.,Brzuszkiewicz A, Nowak E, Dauter Z, Dauter M, Cieslinski H, Dlugolecka A, Kur J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Sep 1;65(Pt, 9):862-5. Epub 2009 Aug 20. PMID:19724118<ref>PMID:19724118</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_19724118}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3hp4" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19724118 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19724118}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3HP4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudoalteromonas_sp._643a Pseudoalteromonas sp. 643a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HP4 OCA].
[[Category: Pseudoalteromonas sp. 643A]]
 
[[Category: Brzuszkiewicz A]]
==Reference==
[[Category: Cieslinski H]]
<ref group="xtra">PMID:19724118</ref><references group="xtra"/>
[[Category: Dauter M]]
[[Category: Pseudoalteromonas sp. 643a]]
[[Category: Dauter Z]]
[[Category: Brzuszkiewicz, A.]]
[[Category: Kur J]]
[[Category: Cieslinski, H.]]
[[Category: Nowak E]]
[[Category: Dauter, M.]]
[[Category: Dauter, Z.]]
[[Category: Kur, J.]]
[[Category: Nowak, E.]]
[[Category: Esterase]]
[[Category: Hydrolase]]
[[Category: Monoethylphosphonate]]
[[Category: Psychrotrophic]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:27:49 2009''
Retrieved from "https://proteopedia.org/3hp4"